Regional expression differences of SERT and TSPAN8 in hippocampus, cerebellum and cortex of wild-type young, adult and middle-aged rats

Hatice Yıldırım; Burcu Efe Daşkan; Elif Aksöz; Fazilet Şen; Murat Çelebi

doi:10.1016/j.gene.2023.147706

Regional expression differences of SERT and TSPAN8 in hippocampus, cerebellum and cortex of wild-type young, adult and middle-aged rats

Gene. 2023 Nov 15:885:147706. doi: 10.1016/j.gene.2023.147706. Epub 2023 Aug 10.

Authors

Hatice Yıldırım¹, Burcu Efe Daşkan², Elif Aksöz³, Fazilet Şen³, Murat Çelebi⁴

Affiliations

¹ Balikesir University, Faculty of Science and Letters, Department of Molecular Biology and Genetics, Cagis Campus, Balikesir, Turkey. Electronic address: hbozkurt@balikesir.edu.tr.
² Balikesir University, Faculty of Science and Letters, Department of Molecular Biology and Genetics, Cagis Campus, Balikesir, Turkey.
³ Balikesir University, Faculty of Medicine, Department of Medical Pharmacology, Cagis Campus, Balikesir, Turkey.
⁴ Balikesir University Savastepe Vocational School, Department of Veterinary Medicine, Savastepe Balikesir, Turkey.

PMID: 37572802
DOI: 10.1016/j.gene.2023.147706

Abstract

Changes in gene expression with aging are associated with a decline in physical and cognitive abilities. Here, we investigated the changes in mRNA and protein expression of TSPAN8 and SERT in the different parts of the brain for different age group rats. Our protein analysis revealed that aging mainly triggers SERT gene expression in the cerebellum and hippocampus, showing that an increase in mRNA expression correlates with protein expression. For TSPAN8, age-dependent protein increase was observed in the hippocampus and highest expression was observed for adult and middle-aged rats.

Keywords: Brain; Rat; SERT; TSPAN8.

MeSH terms

Aging / genetics
Aging / metabolism
Animals
Brain* / metabolism
Cerebellum / metabolism
Hippocampus* / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
Rats

Substances

RNA, Messenger