Novel SERS biosensor for rapid detection of breast cancer based on Ag2O-Ag-PSi nanochips

Xuehua Wang; Cheng Chen; Chen Chen; Enguang Zuo; Shibin Han; Jie Yang; Ziwei Yan; Xiaoyi Lv; Junwei Hou; Zhenhong Jia

doi:10.1016/j.saa.2023.123226

Novel SERS biosensor for rapid detection of breast cancer based on Ag₂O-Ag-PSi nanochips

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 15:303:123226. doi: 10.1016/j.saa.2023.123226. Epub 2023 Aug 2.

Authors

Xuehua Wang¹, Cheng Chen², Chen Chen³, Enguang Zuo³, Shibin Han¹, Jie Yang³, Ziwei Yan², Xiaoyi Lv⁴, Junwei Hou⁵, Zhenhong Jia⁶

Affiliations

¹ College of Physical Science and Technology, Xinjiang University, Urumqi 830046, China.
² College of Software, Xinjiang University, Urumqi 830046, China.
³ College of Information Science and Engineering, Xinjiang University, Urumqi 830046, China.
⁴ College of Software, Xinjiang University, Urumqi 830046, China. Electronic address: xjuwawj01@163.com.
⁵ State Key Laboratory of Heavy Oil Processing, China University of Petroleum-Beijing at Karamay, Karamay 834000, China. Electronic address: Junwhou@126.com.
⁶ College of Information Science and Engineering, Xinjiang University, Urumqi 830046, China. Electronic address: jzhh@xju.edu.cn.

PMID: 37567026
DOI: 10.1016/j.saa.2023.123226

Abstract

Ag₂O-Ag-PSi (porous silicon) surface-enhanced Raman scattering (SERS) chip was successfully synthesized by electrochemical corrosion, in situ reduction and heat treatment technology. The influence of different heat treatment temperature on SERS performance of the chip is studied. The results show that the chip treated at 300 °C has the best SERS performance. The chip was composed of Ag₂O-Ag nano core shell with a diameter of 40-60 nm and porous silicon substrate. Then, the optimized chip was used to perform SERS test on serum samples from 30 healthy volunteers and 30 early breast cancer patients, and the baseline was corrected by LabSpec6 software. Finally, the data were analyzed by principal component analysis combined with t-distributed Stochastic Neighbor Embedding (PCA-t-SNE). The results showed that the accuracy of the improved substrate combined with multivariate statistical method was 98%. The shelf life of the chips exceeded six months due to the presence of the Ag₂O shell. This study provides a basis for developing a low-cost rapid and sensitive early screening technology for breast cancer.

Keywords: Ag(2)O-Ag-PSi Chip; Breast cancer (BRC); Rapid detection; Surface-enhanced Raman scattering (SERS).

MeSH terms

Biosensing Techniques*
Breast Neoplasms* / diagnosis
Female
Humans
Metal Nanoparticles*
Silicon
Silver
Spectrum Analysis, Raman / methods

Substances

Silicon
Silver