Insight into differences in whey proteome from human and eight dairy animal species for formula humanization

Zhongyuan Ji; Ruifeng Dong; Qijing Du; Hongning Jiang; Rongbo Fan; Dengpan Bu; Jun Wang; Zhongna Yu; Rongwei Han; Yongxin Yang

doi:10.1016/j.foodchem.2023.137076

Insight into differences in whey proteome from human and eight dairy animal species for formula humanization

Food Chem. 2024 Jan 1:430:137076. doi: 10.1016/j.foodchem.2023.137076. Epub 2023 Aug 2.

Authors

Zhongyuan Ji¹, Ruifeng Dong², Qijing Du¹, Hongning Jiang³, Rongbo Fan⁴, Dengpan Bu⁵, Jun Wang⁶, Zhongna Yu¹, Rongwei Han⁷, Yongxin Yang⁸

Affiliations

¹ College of Food Science and Engineering, Shandong Technology Innovation Center of Special Food, Qingdao Agricultural University, Qingdao 266109, China; Qingdao Special Food Research Institute, Qingdao 266109, China.
² Department of Obstetrics, Qingdao Municipal Hospital, Qingdao 266001, China.
³ College of Food Science and Engineering, Shandong Technology Innovation Center of Special Food, Qingdao Agricultural University, Qingdao 266109, China; Qingdao Special Food Research Institute, Qingdao 266109, China. Electronic address: jhn2019@qau.edu.cn.
⁴ College of Food Science and Engineering, Shandong Technology Innovation Center of Special Food, Qingdao Agricultural University, Qingdao 266109, China; Qingdao Special Food Research Institute, Qingdao 266109, China. Electronic address: fanrongbo@qau.edu.cn.
⁵ College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China. Electronic address: budengpan@qau.edu.cn.
⁶ College of Food Science and Engineering, Shandong Technology Innovation Center of Special Food, Qingdao Agricultural University, Qingdao 266109, China.
⁷ College of Food Science and Engineering, Shandong Technology Innovation Center of Special Food, Qingdao Agricultural University, Qingdao 266109, China; Qingdao Special Food Research Institute, Qingdao 266109, China. Electronic address: qauhan@qau.edu.cn.
⁸ College of Food Science and Engineering, Shandong Technology Innovation Center of Special Food, Qingdao Agricultural University, Qingdao 266109, China; Qingdao Special Food Research Institute, Qingdao 266109, China. Electronic address: yangyx1216@126.com.

PMID: 37566980
DOI: 10.1016/j.foodchem.2023.137076

Abstract

Human breastmilk fulfills the nutritional needs of infants and therefore is the best template for formula. In this study, whey proteins were investigated among human and eight dairy animal species using label-free proteomics approach. Totally, 965 proteins from milk whey were identified and large variations were observed between human and animals. Several proteins, including β-galactosidase, fatty acid synthase, osteopontin, lactoferrin, mannose receptor, and complement C4-A, which are associated with digestion and immune response, exhibited significantly higher levels in human milk whey. Conversely, specific animal milk whey demonstrated elevated abundance of lipocalin 2, lysozyme, and glycosylation-dependent cell adhesion molecule 1. These differential proteins are enriched in complement and coagulation cascades, lysosome, and phagosome pathways. The findings shed light on the variations in the whey proteome composition between human and animal milk, which can contribute to optimizing formula humanization.

Keywords: Dairy animal milk; Human milk; Humanization; Proteomics.

MeSH terms

Animals
Food, Formulated
Humans
Milk / metabolism
Milk Proteins / metabolism
Milk, Human / metabolism
Proteome*
Whey Proteins
Whey* / metabolism

Substances

Milk Proteins
Proteome
Whey Proteins