Antimicrobial susceptibility testing to evaluate minimum inhibitory concentration values of clinically relevant antibiotics

Lucien Barnes V; Douglas M Heithoff; Scott P Mahan; John K House; Michael J Mahan

doi:10.1016/j.xpro.2023.102512

Antimicrobial susceptibility testing to evaluate minimum inhibitory concentration values of clinically relevant antibiotics

STAR Protoc. 2023 Sep 15;4(3):102512. doi: 10.1016/j.xpro.2023.102512. Epub 2023 Aug 10.

Authors

Lucien Barnes V¹, Douglas M Heithoff², Scott P Mahan³, John K House⁴, Michael J Mahan⁵

Affiliations

¹ Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA 93106, USA. Electronic address: lucienbarnes@ucsb.edu.
² Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA 93106, USA.
³ Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA 93106, USA; Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, CA 95616, USA.
⁴ Faculty of Science, School of Veterinary Science, The University of Sydney, Camden, NSW 2570, Australia.
⁵ Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA 93106, USA. Electronic address: mahan@ucsb.edu.

Abstract

Antimicrobial susceptibility testing is used to determine the minimum inhibitory concentration (MIC), the standard measurement of antibiotic activity. Here, we present a protocol for evaluating MIC values of clinically relevant antibiotics against bacterial isolates cultured in standard bacteriologic medium and in mammalian cell culture medium. We describe steps for pathogen identification, culturing bacteria, preparing MIC plates, MIC assay incubation, and determining MIC. This protocol can potentially optimize the use of existing antibiotics while enhancing efforts to discover new ones. For complete details on the use and execution of this protocol, please refer to Heithoff et al.¹.

Keywords: Clinical Protocol; Health Sciences; Microbiology.

MeSH terms

Animals
Anti-Bacterial Agents* / pharmacology
Bacteria*
Mammals
Microbial Sensitivity Tests

Substances

Anti-Bacterial Agents

Grants and funding

P01 HL131474/HL/NHLBI NIH HHS/United States