Transcriptomic analysis of humic acid in relieving the inhibitory effect of high nitrogen on soybean nodulation

Wenhua Zhang; Jia Li; Hongya Li; Dongdong Zhang; Baocheng Zhu; Hongli Yuan; Tongguo Gao

doi:10.3389/fpls.2023.1196939

Transcriptomic analysis of humic acid in relieving the inhibitory effect of high nitrogen on soybean nodulation

Front Plant Sci. 2023 Jul 26:14:1196939. doi: 10.3389/fpls.2023.1196939. eCollection 2023.

Authors

Wenhua Zhang¹, Jia Li¹, Hongya Li¹, Dongdong Zhang¹, Baocheng Zhu¹, Hongli Yuan², Tongguo Gao¹

Affiliations

¹ Hebei Engineering Research Center for Resource Utilization of Agricultural Waste, College of Life Sciences, Hebei Agricultural University, Baoding, China.
² State Key Laboratory of Agrobiotechnology and Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China.

Abstract

Introduction: Nitrogen fertilizer intake promotes soybean growth before the formation of nodules, but excess nitrogen has an inhibitory effect on soybean nodulation. It is important to balance nitrogen levels to meet both growth and nodulation needs.

Methods: the nitrogen level suitable for soybean growth and nodulation was studied, the role of humic acid (HA) in alleviating the inhibition of high nitrogen on soybean nodulation was analyzed, and transcriptomic analysis was performed to understand its mechanism.

Results: The results showed that a lower level of nitrogen with 36.4 mg urea per pot could increase the number of nodules of soybean, and a higher level of nitrogen with 145.9 mg urea per pot (U4 group) had the best growth indicators but inhibited nodulation significantly. HA relieved the inhibitory effect at high nitrogen level, and the number of nodules increased by 122.1% when 1.29 g HA was added (H2 group) compared with the U4 group. The transcriptome analysis was subsequently performed on the H2 and U4 groups, showing that there were 2995 differentially expressed genes (DEGs) on the 25th day, accounting for 6.678% of the total annotated genes (44,848) under the test conditions. These DEGs were enriched in mitogen-activated protein kinase signaling pathway-plant, flavonoid biosynthesis, and plant hormone signal transduction based on the -log10 (P _adjusted) value in the Kyoto Encyclopedia of Genes and Genomes pathway (KEGG).

Discussion: HA balanced the nitrogen level through the above pathways in soybean planting to control the number of nodules.

Keywords: high nitrogen; humic acid; nodulation; relieve inhibition; transcriptome.

Grants and funding

This work was supported by the Natural Science Foundation of Hebei Province (C2021204053), National Natural Science Foundation of China (41807037 and 31770541) and Key R & D projects in Hebei Province (20322907D).