Vitamin A regulates dermal papilla cell proliferation and apoptosis under heat stress via IGF1 and Wnt10b signaling

Zhengkai Yue; Mengqi Liu; Bin Zhang; Fan Li; Chenyang Li; Xiaoyang Chen; Fuchang Li; Lei Liu

doi:10.1016/j.ecoenv.2023.115328

Vitamin A regulates dermal papilla cell proliferation and apoptosis under heat stress via IGF1 and Wnt10b signaling

Ecotoxicol Environ Saf. 2023 Aug 8:262:115328. doi: 10.1016/j.ecoenv.2023.115328. Online ahead of print.

Authors

Zhengkai Yue¹, Mengqi Liu¹, Bin Zhang¹, Fan Li¹, Chenyang Li¹, Xiaoyang Chen¹, Fuchang Li², Lei Liu³

Affiliations

¹ Key Laboratory of Efficient Utilization of Non-grain Feed Resources (Co-construction by Ministry and Prov-ince), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Biotechnol-ogy and Disease Control and Prevention, Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271018, China.
² Key Laboratory of Efficient Utilization of Non-grain Feed Resources (Co-construction by Ministry and Prov-ince), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Biotechnol-ogy and Disease Control and Prevention, Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271018, China. Electronic address: chlf@sdau.edu.cn.
³ Key Laboratory of Efficient Utilization of Non-grain Feed Resources (Co-construction by Ministry and Prov-ince), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Biotechnol-ogy and Disease Control and Prevention, Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271018, China. Electronic address: leiliu@sdau.edu.cn.

PMID: 37562175
DOI: 10.1016/j.ecoenv.2023.115328

Abstract

Heat stress (HS) negatively affects the development of hair follicles. The present study investigated the effect of vitamin A (VA) on the development of rabbit dermal papilla cells (DPCs) under HS and the underlying regulatory mechanisms. Addition of 0.4 mg/L VA to the culture medium significantly enhanced cell proliferation (P < 0.001) and inhibited the apoptosis of DPCs (P < 0.01). VA decreased the proportion of DPCs in G0/G1 stage of the cell cycle under HS along with the expression of caspase 3, heat shock protein 70 (HSP70), and microRNA 195 (miR-195) (P < 0.05). VA also activated the insulin-like growth factor 1 (IGF1) and Wnt10b/β-catenin signaling pathways. The results of the dual luciferase reporter assay showed that IGF1 expression was modulated by miR-195-5p. Over-expression of miR-195-5p in DPCs with HS+VA treatment significantly reduced cell viability and IGF1 signaling (P < 0.01) and increased apoptosis (P < 0.01) compared with the HS+VA group. The positive effects of VA on proliferation and apoptosis of DPCs under HS were significantly attenu-ated by blocking Wnt10b and β-catenin signaling with IWP-2 and XAV-939, respectively. These results demonstrate that VA can promote hair follicle development following HS via modulation of miR-195/IGF1 and Wnt10b/β-catenin signaling pathways.

Keywords: Dermal papilla cells; Heat stress; Signaling pathways; Vitamin A.