Combination of metabolome and proteome analyses provides insights into the mechanism underlying growth differences in Acipenser dabryanus

Xiaoyun Wu; Jiansheng Lai; Yeyu Chen; Ya Liu; Mingjiang Song; Feiyang Li; Pengcheng Li; Qingzhi Li; Quan Gong

doi:10.1016/j.isci.2023.107413

Combination of metabolome and proteome analyses provides insights into the mechanism underlying growth differences in Acipenser dabryanus

iScience. 2023 Jul 18;26(8):107413. doi: 10.1016/j.isci.2023.107413. eCollection 2023 Aug 18.

Authors

Xiaoyun Wu^{1

2}, Jiansheng Lai^{1

2}, Yeyu Chen^{1

2}, Ya Liu^{1

2}, Mingjiang Song^{1

2}, Feiyang Li^{1

2}, Pengcheng Li^{1

2}, Qingzhi Li^{1

2}, Quan Gong^{1

2}

Affiliations

¹ The Fishery Institute of the Sichuan Academy of Agricultural Sciences, Chengdu 611730, China.
² Fish Resources and Environment in the Upper Reaches of the Yangtze River Observation and Research Station of Sichuan Province, Chengdu 611730, China.

Abstract

To analyze the differences between different-sized Acipenser dabryanus, we randomly selected 600 3-month-old A. dabryanus juveniles. Four months later, the blood and white muscle of these fish were analyzed. The results showed no significant difference in the length-weight relationship (LWR) b value between the large and small A. dabryanus. The levels of serum growth hormone (gh) and insulin-like growth factor 1 (igf1) in the large A. dabryanus were significantly lower than those in the small, whereas the activity levels of Total superoxide dismutase (T-sod) and catalase (cat) were opposite to the results of gh and igf1. A total of 212 and 245 metabolites showed significant changes in the positive and negative polarity mode, respectively. Among 3,308 proteins identified, 69 proteins showed upregulated expression, and 185 proteins showed downregulated expression. These results indicated that the growth advantage of A. dabryanus was closely related to glycolysis, protein synthesis, and antioxidant function.

Keywords: Ichthyology; Metabolomics; Proteomics.