Reporter gene assays are critical tools of nuclear receptor research for characterizing the effects of ligands on nuclear receptor activity. Common luciferase-based techniques require expensive substrates and are typically performed in endpoint format. Here, we describe a versatile reporter gene assay to observe nuclear receptor activity with fluorescent proteins as reporters. This setting is highly cost-efficient and enables observation of nuclear receptor activity over time with multiple measurements from one plate.
Keywords: Cost-efficient; Fluorescent proteins; Luciferase; Time-resolved activity.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.