ZNF671 methylation test in cervical scrapings for cervical intraepithelial neoplasia grade 3 and cervical cancer detection

Peng Zhu; Jing Xiong; Ding Yuan; Xiang Li; Lili Luo; Ju Huang; Binbin Wang; Quanfang Nie; Shuli Wang; Liying Dang; Yan Chen; Shu Li; Yuhang An; Li Yang; Rong Liu; Yanping He; Xiong Li; Puxiang Chen; Honghao Zhou; Qing Li

doi:10.1016/j.xcrm.2023.101143

ZNF671 methylation test in cervical scrapings for cervical intraepithelial neoplasia grade 3 and cervical cancer detection

Cell Rep Med. 2023 Aug 15;4(8):101143. doi: 10.1016/j.xcrm.2023.101143. Epub 2023 Aug 8.

Authors

Peng Zhu¹, Jing Xiong², Ding Yuan³, Xiang Li⁴, Lili Luo⁵, Ju Huang⁵, Binbin Wang⁶, Quanfang Nie⁶, Shuli Wang⁷, Liying Dang⁷, Yan Chen⁸, Shu Li⁸, Yuhang An⁸, Li Yang⁸, Rong Liu¹, Yanping He⁹, Xiong Li⁹, Puxiang Chen², Honghao Zhou¹, Qing Li¹⁰

Affiliations

¹ Department of Clinical Pharmacology, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha 410008, P.R. China; Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, 110 Xiangya Road, Changsha 410078, P.R. China; Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, 110 Xiangya Road, Changsha 410078, P.R. China.
² Department of Gynaecology and Obstetrics, The Second Xiangya Hospital, Central South University, 139 Renming Road, Changsha 410011, P.R. China.
³ Health Management Center, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha 410008, P.R. China.
⁴ Department of Gynaecology, The Third Xiangya Hospital, Central South University, 138 Tongzipo Road, Changsha 410013, P.R. China.
⁵ Department of Gynaecology, The First Affiliated Hospital of Shantou University Medical College, Shantou, P.R. China.
⁶ Department of Obstetrics and Gynecology, Loudi Central Hospital, 51 Chang Qing Road, Loudi 417000, P.R. China.
⁷ Department of Obstetrics and Gynecology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450000, P.R. China.
⁸ Xiangya Medical Laboratory, Central South University, 110 Xiangya Road, Changsha 410078, P.R. China.
⁹ Key Specialty of Clinical Pharmacy, The First Affiliated Hospital, Guangdong Pharmaceutical University, 19 Nonglinxia Road, Yuexiu District, Guangzhou, Guangdong, P.R. China.
¹⁰ Department of Clinical Pharmacology, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha 410008, P.R. China; Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, 110 Xiangya Road, Changsha 410078, P.R. China; Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, 110 Xiangya Road, Changsha 410078, P.R. China. Electronic address: liqing9251026@csu.edu.cn.

Abstract

Effective triage of high-risk human papillomavirus (hrHPV)+ women is warranted to avoid unnecessary referral and overtreatment. Molecular triage tests have recently begun to impact cervical intraepithelial neoplasia grade 3 (CIN3) or cervical cancer (CC), termed CIN3+, detection. We find that zinc finger protein 671 methylation (ZNF671^m) test has superior performance for CIN3+ detection in all single molecular triage tests, including HPV16/18 genotyping, paired box gene 1 methylation (PAX1^m), and ZNF671^m, in the training set. Using ZNF671^m test instead of Thinprep cytologic test (TCT) as a single triage strategy or as a combined triage strategy with HPV16/18 genotyping has achieved comparable sensitivity but higher specificity for CIN3+ detection among 391 hrHPV+ women in the validation set. Little attention has been paid to the women with hrHPV- status but detected CIN3+. We find that the CIN3+ risk after a negative result could be reduced further by triage using ZNF671^m in hrHPV- patients.

Keywords: ASCUS; CIN3+ detection; DNA methylation; Thinprep cytologic test; ZNF671; cervical cancer; hrHPV.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA Methylation / genetics
Female
Human papillomavirus 16 / genetics
Human papillomavirus 18 / genetics
Humans
Tumor Suppressor Proteins / genetics
Uterine Cervical Dysplasia* / diagnosis
Uterine Cervical Neoplasms* / diagnosis
Uterine Cervical Neoplasms* / genetics

Substances

ZNF671 protein, human
Tumor Suppressor Proteins