BrMYB108 confers resistance to Verticillium wilt by activating ROS generation in Brassica rapa

Tongbing Su; Weihong Wang; Zheng Wang; Peirong Li; Xiaoyun Xin; Yangjun Yu; Deshuang Zhang; Xiuyun Zhao; Jiao Wang; Liling Sun; Guihua Jin; Fenglan Zhang; Shuancang Yu

doi:10.1016/j.celrep.2023.112938

BrMYB108 confers resistance to Verticillium wilt by activating ROS generation in Brassica rapa

Cell Rep. 2023 Aug 29;42(8):112938. doi: 10.1016/j.celrep.2023.112938. Epub 2023 Aug 6.

Authors

Affiliations

¹ State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, China.
² State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China.
³ State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, China. Electronic address: zhangfenglan@nercv.org.
⁴ State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, China. Electronic address: yushuancang@nercv.org.

PMID: 37552600
DOI: 10.1016/j.celrep.2023.112938

Abstract

Increasing plant resistance to Verticillium wilt (VW), which causes massive losses of Brassica rapa crops, is a challenge worldwide. However, few causal genes for VW resistance have been identified by forward genetic approaches, resulting in limited application in breeding. We combine a genome-wide association study in a natural population and quantitative trait locus mapping in an F2 population and identify that the MYB transcription factor BrMYB108 regulates plant resistance to VW. A 179 bp insertion in the BrMYB108 promoter alters its expression pattern during Verticillium longisporum (VL) infection. High BrMYB108 expression leads to high VL resistance, which is confirmed by disease resistance tests using BrMYB108 overexpression and loss-of-function mutants. Furthermore, we verify that BrMYB108 confers VL resistance by regulating reactive oxygen species (ROS) generation through binding to the promoters of respiratory burst oxidase genes (Rboh). A loss-of-function mutant of AtRbohF in Arabidopsis shows significant susceptibility to VL. Thus, BrMYB108 and its target ROS genes could be used as targets for genetic engineering for VL resistance of B. rapa.

Keywords: BrMYB108; Brassica rapa; CP: Plants; ROS; Rboh; Verticillium.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Brassica rapa* / genetics
Disease Resistance / genetics
Genome-Wide Association Study
Plant Breeding
Plant Diseases / genetics
Reactive Oxygen Species
Verticillium* / genetics

Substances

Reactive Oxygen Species

Supplementary concepts

Verticillium longisporum