Hydrothermal liquid digestate has been widely accepted as a substrate in anaerobic digestion (AD) for energy recovery. However, the potential negative impacts of hydrothermal liquid digestate on AD remain unclear. In this study, the organic biodegradability of hydrothermal liquid digestate produced from hydrothermal treatment (HTT) at different temperatures was analyzed, and the formation and degradation process of potential inhibitory substances were discussed. Results demonstrated that the AD lag phase of hydrothermal liquid digestate increased from 3 days at raw liquid digestate to 5-21 days. When the HTT temperature reached 220 °C, the methane yield decreased by 48%, and more than 71% of the organics in the hydrothermal liquid digestate were not utilized by AD. Biorefractory substances, such as fulvic and humic acids, accumulate in the hydrothermal liquid digestate. Potential inhibitory substances from Maillard reactions mainly affect the methanogenesis of AD. Most inhibitory substances were degraded within 7-22 days, with the degradation rate following the order of pyrroles > pyrazines > ketones > imidazoles > indoles. The AD community structure and methane conversion were partially re-established after most inhibitory substances were degraded. This study provides valuable information on eliminating the potential negative effects of hydrothermal liquid digestate on AD.
Keywords: Biorefractory substances; Food waste digestate; Hydrothermal liquid; Inhibitors degradation; Inhibitory mechanism; Organic conversion.
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