Discrimination of toxigenic and non-toxigenic Aspergillus flavus in wheat based on nanocomposite colorimetric sensor array

Abstract

A novel method was developed for the early detection of wheat infected with Aspergillus flavus (A. flavus) using a nanocomposite colorimetric sensors array (CSA). LC-MS analysis revealed the presence of Aflatoxin B1 (AFB1) and Aflatoxin B2 (AFB2) on day seven, demonstrating mycotoxin variabilities in infected wheat. HS-SPME-GC-MS analysis detected 2-methylbutyral, a gas exclusively associated with toxigenic A. flavus. The CSA was modified using three nanoparticles of MOF and successfully used to detect the wheat infected with A. flavus. Discrimination of different types of infected wheat samples was achieved using the RGB difference map and Principal Component Analysis (PCA) model. Additionally, the Linear Discriminant Analysis (LDA) model accurately predicted the presence of toxigenic A. flavus at various stages of infection. These findings highlight the promising capabilities of nanocomposite CSA for early-stage detection of A. flavus infection in wheat.

Keywords: Toxigenic and non-toxigenic Aspergillus flavus; Volatile organic compounds; Wheat nanocomposite colorimetric sensors array.