Integrating 4-D light-sheet fluorescence microscopy and genetic zebrafish system to investigate ambient pollutants-mediated toxicity

Sheila Gonzalez-Ramos; Jing Wang; Jae Min Cho; Enbo Zhu; Seul-Ki Park; Julie G In; Srinivasa T Reddy; Eliseo F Castillo; Matthew J Campen; Tzung K Hsiai

doi:10.1016/j.scitotenv.2023.165947

Integrating 4-D light-sheet fluorescence microscopy and genetic zebrafish system to investigate ambient pollutants-mediated toxicity

Sci Total Environ. 2023 Dec 1:902:165947. doi: 10.1016/j.scitotenv.2023.165947. Epub 2023 Aug 3.

Authors

Affiliations

¹ Division of Cardiology, Department of Medicine, David Geffen School of Medicine at University of California, Los Angeles, CA, USA; Department of Bioengineering, School of Engineering & Applied Science, University of California, Los Angeles, CA, USA.
² Department of Bioengineering, School of Engineering & Applied Science, University of California, Los Angeles, CA, USA.
³ Division of Cardiology, Department of Medicine, David Geffen School of Medicine at University of California, Los Angeles, CA, USA.
⁴ Division of Gastroenterology and Hepatology, Department of Internal Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.
⁵ Division of Cardiology, Department of Medicine, David Geffen School of Medicine at University of California, Los Angeles, CA, USA; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, USA; Molecular Toxicology Interdepartmental Degree Program, Fielding School of Public Health, University of California, Los Angeles, CA, USA.
⁶ Department of Pharmaceutical Sciences, College of Pharmacy, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.
⁷ Division of Cardiology, Department of Medicine, David Geffen School of Medicine at University of California, Los Angeles, CA, USA; Department of Bioengineering, School of Engineering & Applied Science, University of California, Los Angeles, CA, USA; Greater Los Angeles VA Healthcare System, Department of Medicine, Los Angeles, California, USA. Electronic address: Thsiai@mednet.ucla.edu.

Abstract

Ambient air pollutants, including PM_2.5 (aerodynamic diameter d ~2.5 μm), PM₁₀ (d ~10 μm), and ultrafine particles (UFP: d < 0.1 μm) impart both short- and long-term toxicity to various organs, including cardiopulmonary, central nervous, and gastrointestinal systems. While rodents have been the principal animal model to elucidate air pollution-mediated organ dysfunction, zebrafish (Danio rerio) is genetically tractable for its short husbandry and life cycle to study ambient pollutants. Its electrocardiogram (ECG) resembles that of humans, and the fluorescent reporter-labeled tissues in the zebrafish system allow for screening a host of ambient pollutants that impair cardiovascular development, organ regeneration, and gut-vascular barriers. In parallel, the high spatiotemporal resolution of light-sheet fluorescence microscopy (LSFM) enables investigators to take advantage of the transparent zebrafish embryos and genetically labeled fluorescent reporters for imaging the dynamic cardiac structure and function at a single-cell resolution. In this context, our review highlights the integrated strengths of the genetic zebrafish system and LSFM for high-resolution and high-throughput investigation of ambient pollutants-mediated cardiac and intestinal toxicity.

Keywords: Cardiovascular health; Gastrointestinal health; Light-sheet fluorescence microscopy; Ultrafine particles; Zebrafish.

Publication types

Review

MeSH terms

Air Pollutants* / analysis
Air Pollutants* / toxicity
Air Pollution* / analysis
Animals
Environmental Pollutants*
Humans
Microscopy, Fluorescence / methods
Particulate Matter / toxicity
Zebrafish

Substances

Environmental Pollutants
Air Pollutants
Particulate Matter

Abstract

Publication types

MeSH terms

Substances

Grants and funding