Hp0521 inhibited the virulence of H. pylori 26,695 strain via regulating CagA expression

Min Yu; Min Xu; Yixin Shen; Yun Liu; Chi Xu; Tongbao Feng; Ping Zhang

doi:10.1016/j.heliyon.2023.e17881

Hp0521 inhibited the virulence of H. pylori 26,695 strain via regulating CagA expression

Heliyon. 2023 Jul 1;9(7):e17881. doi: 10.1016/j.heliyon.2023.e17881. eCollection 2023 Jul.

Authors

Min Yu¹, Min Xu¹, Yixin Shen², Yun Liu², Chi Xu³, Tongbao Feng¹, Ping Zhang¹

Affiliations

¹ Department of Clinical Laboratory, The Affiliated Changzhou Second People's Hospital of Nanjing Medical University, Changzhou, 213000, China.
² Department of Medical Microbiology, School of Medicine, Jiangsu University, Zhenjiang, 212013, China.
³ Digestive Disease Center, Shanghai East Hospital, Tongji University, Shanghai, 200120, China.

Abstract

Hp0521 is the number of cag pathogenicity island (cagPAI) family in Helicobacter pylori (H. pylori, Hp), which encoded Cag2 protein. The aim of this study was to investigate the role of hp0521 on the H. pylori 26,695 strain. We constructed the recombinant prokaryotic expression plasmid pET-32a-hp0521 and pET-32a-hpc0521. Then, we co-cultured the H. pylori wild strain 26,695 and Δhp0521 strain with GES-1 cells to detect CagA protein transport and IL-8 secretion. We found that Δhp0521 mutation increased the expression of cagA, rpoB and promoted the transportation of CagA protein in GES-1 cells. In addition, we also observed that Δhp0521 mutation had no effect on other cagPAI protein stability and the expression of IL-8. Our findings suggested that hp0521 may down-regulated the expression of cagA, rpoB and inhibited the transportation of CagA protein in GES-1 cells and had no effect on growth.

Keywords: Helicobacter pylori; cagA; cagPAI; hp0521.