Seasonal changes in the abundance Fusarium proliferatium, microbial endophytes and nutrient levels in the roots of hybrid bamboo Bambusa pervariabilis × Dendrocalamopsis grandis

Lin Li; Yaxuan Wang; Cailin Yu; Shuying Li; Tiantian Lin; Shan Han; Tianhui Zhu; Shujiang Li

doi:10.3389/fpls.2023.1185449

Seasonal changes in the abundance Fusarium proliferatium, microbial endophytes and nutrient levels in the roots of hybrid bamboo Bambusa pervariabilis × Dendrocalamopsis grandis

Front Plant Sci. 2023 Jul 19:14:1185449. doi: 10.3389/fpls.2023.1185449. eCollection 2023.

Authors

Lin Li¹, Yaxuan Wang¹, Cailin Yu¹, Shuying Li¹, Tiantian Lin¹, Shan Han¹, Tianhui Zhu¹, Shujiang Li^{1

2}

Affiliations

¹ College of Forestry, Sichuan Agricultural University, Chengdu, China.
² National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River, Chengdu, China.

Abstract

Plant root pathogens invade the soil around plant roots, disturbing the systemic balance, reducing plant defenses, and causing severe disease. At present, there are few studies on the severity of plant diseases caused by pathogen invasion in different seasons and how pathogens affect root microecology. In this study, we compared the levels of nutrients in the root tissues of the two groups of plants. We used 16S and ITS amplicon sequencing with Illumina NovaSeq 6000 to compare seasonal changes in the composition and structure of microbial communities from healthy roots of bamboo Bambusa pervariabilis × Dendrocalamopsis grandis and roots infected by the soilborne pathogen Fusarium proliferatum. We have found that the invasion of the pathogen led to a substantial decrease in nutrient elements in bamboo roots, except for nitrogen. The pathogen presence correlated with seasonal changes in the bamboo root microbiome and decreased bacterial richness in diseased plants. The root microbial community structure of healthy plants was more stable than that of their diseased counterparts. Furthermore, we identified the lesion area and relative abundance of F. proliferatum were significant predictors of disease progression. The potassium tissue content and the disease lesion area were identified as factors linked with the observed changes in the bamboo root microbiome. This study provides a theoretical foundation for understanding the seasonal dynamics F. proliferatum, an economically important soilborne pathogen of hybrid bamboo grown in Sichuan Province, China.

Keywords: bamboo; basal rot; root microorganism; root nutrient elements; seasonal variation.

Grants and funding

This research was funded by the National Natural Science Foundation of China, grant number 32171795; the Sichuan Natural Science Foundation for Distinguished Young Scholar, grant number 2023NSFSC1936; the Innovation Training Program for College Students, grant number S202310626075.