Cellulose microcrystalline: A promising ecofriendly approach to control Culex quinquefasciatus larvae

Sindoval Silva de Souza; Alex Rodrigues Gomes; Abraão Tiago Batista Guimarães; Aline Sueli de Lima Rodrigues; Letícia Paiva de Matos; Juliana Dos Santos Mendonça; Thiarlen Marinho da Luz; Stênio Gonçalves da Silva Matos; M Safiur Rahman; Chinnasamy Ragavendran; Sengottayan Senthil-Nathan; Ajay Guru; Md Refat Jahan Rakib; Nabisab Mujawar Mubarak; Md Mostafizur Rahman; Thiago Lopes Rocha; Abu Reza Md Towfiqul Islam; Guilherme Malafaia

doi:10.1016/j.scitotenv.2023.165952

Cellulose microcrystalline: A promising ecofriendly approach to control Culex quinquefasciatus larvae

Sci Total Environ. 2023 Nov 25:901:165952. doi: 10.1016/j.scitotenv.2023.165952. Epub 2023 Aug 1.

Authors

Sindoval Silva de Souza¹, Alex Rodrigues Gomes², Abraão Tiago Batista Guimarães³, Aline Sueli de Lima Rodrigues⁴, Letícia Paiva de Matos³, Juliana Dos Santos Mendonça³, Thiarlen Marinho da Luz⁵, Stênio Gonçalves da Silva Matos³, M Safiur Rahman⁶, Chinnasamy Ragavendran⁷, Sengottayan Senthil-Nathan⁸, Ajay Guru⁹, Md Refat Jahan Rakib¹⁰, Nabisab Mujawar Mubarak¹¹, Md Mostafizur Rahman¹², Thiago Lopes Rocha¹³, Abu Reza Md Towfiqul Islam¹⁴, Guilherme Malafaia¹⁵

Affiliations

¹ Post-Graduation Program in Biotechnology and Biodiversity, Federal University of Goiás, Goiânia, GO, Brazil; Laboratory of Toxicology Applied to the Environment, Goiano Federal Institute - Urutaí Campus. Rodovia Geraldo Silva Nascimento, 2,5 km, Zona Rural, Urutaí, GO, Brazil.
² Laboratory of Toxicology Applied to the Environment, Goiano Federal Institute - Urutaí Campus. Rodovia Geraldo Silva Nascimento, 2,5 km, Zona Rural, Urutaí, GO, Brazil; Post-Graduation Program in Ecology, Conservation, and Biodiversity, Federal University of Uberlândia, Uberlândia, MG, Brazil.
³ Laboratory of Toxicology Applied to the Environment, Goiano Federal Institute - Urutaí Campus. Rodovia Geraldo Silva Nascimento, 2,5 km, Zona Rural, Urutaí, GO, Brazil.
⁴ Laboratory of Toxicology Applied to the Environment, Goiano Federal Institute - Urutaí Campus. Rodovia Geraldo Silva Nascimento, 2,5 km, Zona Rural, Urutaí, GO, Brazil; Post-Graduation Program in Conservation of Cerrado Natural Resources, Goiano Federal Institute, Urutaí, GO, Brazil.
⁵ Post-Graduation Program in Biotechnology and Biodiversity, Federal University of Goiás, Goiânia, GO, Brazil; Laboratory of Toxicology Applied to the Environment, Goiano Federal Institute - Urutaí Campus. Rodovia Geraldo Silva Nascimento, 2,5 km, Zona Rural, Urutaí, GO, Brazil; Post-Graduation Program in Ecology, Conservation, and Biodiversity, Federal University of Uberlândia, Uberlândia, MG, Brazil.
⁶ Department of Environmental Science and Disaster Management, Noakhali Science and Technology University, Noakhali 3814, Bangladesh.
⁷ Saveetha Dental College and Hospitals (SIMATS), Saveetha University Chennai, Tamil Nadu, India.
⁸ Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi, Tirunelveli, Tamil Nadu 627 412, India.
⁹ Department of Cariology, Saveetha Dental College and Hospitals, SIMATS, Chennai 600 077, Tamil Nadu, India.
¹⁰ Noakhali Science and Technology University, Noakhali, Bangladesh.
¹¹ Petroleum and Chemical Engineering, Faculty of Engineering, Universiti Teknologi Brunei, Bandar Seri Begawan BE1410, Brunei Darussalam; Department of Biosciences, Saveetha School of Engineering, Saveetha institute of Medical and Technical Sciences, Chennai, India.
¹² Jahangirnagar University Dhaka-1342, Bangladesh.
¹³ Laboratory of Environmental Biotechnology and Ecotoxicology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, Goiás, Brazil.
¹⁴ Department of Disaster Management, Begum Rokeya University, Rangpur 5400, Bangladesh.
¹⁵ Post-Graduation Program in Biotechnology and Biodiversity, Federal University of Goiás, Goiânia, GO, Brazil; Laboratory of Toxicology Applied to the Environment, Goiano Federal Institute - Urutaí Campus. Rodovia Geraldo Silva Nascimento, 2,5 km, Zona Rural, Urutaí, GO, Brazil; Post-Graduation Program in Ecology, Conservation, and Biodiversity, Federal University of Uberlândia, Uberlândia, MG, Brazil; Post-Graduation Program in Conservation of Cerrado Natural Resources, Goiano Federal Institute, Urutaí, GO, Brazil. Electronic address: guilhermeifgoiano@gmail.com.

PMID: 37536599
DOI: 10.1016/j.scitotenv.2023.165952

Abstract

The growing use of synthetic chemical compounds/substances in vector control of mosquitoes, associated with their adverse effects on the environment and non-target organisms, has demanded the development of eco-friendly alternatives. In this context, this study aimed to evaluate the larvicidal action of different cellulose microcrystalline (CMs) concentrations and investigate their toxicity mechanisms in Culex quinquefasciatus fourth instar larvae as a model species. Probit analysis revealed that the median lethal concentrations (LC₅₀) for 24 h and 36 h exposure were 100 and 58.29 mg/L, respectively. We also showed that such concentrations induced a redox imbalance in the larvae, marked by an increase in the production of reactive oxygen species (ROS) and thiobarbituric acid reactive substances (TBARS), as well as a reduction in the activity of superoxide dismutase (SOD) and catalase (CAT). Furthermore, different alterations in the external morphology of the larvae were associated with the ingestion of CMs. On the other hand, exposure of adult zebrafish (Danio rerio) to LC₅₀24h and LC₅₀36h for seven days did not induce any behavioral changes or alterations mutagenic, genotoxic, biochemical, or in the production of cytokines IFN-γ and IL-10. Thus, taken together, our study demonstrates for the first time that the use of CMs can constitute a promising strategy in the control of C. quinquefasciatus larvae, combining insecticidal efficiency with an "eco-friendly" approach in the fight against an important mosquito vector of several human diseases.

Keywords: Eco-friendly particles; Ecotoxicity; Larvicide assay; Mosquitoes; Vector control; Zebrafish.