Mulberry fruit repairs alcoholic liver injury by modulating lipid metabolism and the expression of miR-155 and PPARα in rats

Funct Integr Genomics. 2023 Aug 2;23(3):261. doi: 10.1007/s10142-023-01131-y.

Abstract

As alcohol consumption increases, alcoholic liver disease (ALD) has become more popular and is threating our human life. In this study, we found mulberry fruit extract (MFE) repaired alcohol-caused liver diseases by regulating hepatic lipid biosynthesis pathway and oxidative singling in alcoholically liver injured (ALI) rats. MFE administration inhibited hepatic lipid accumulation and improved liver steatosis in ALI rats. MFE also enhanced the antioxidant capacity and alleviated the inflammatory response by increasing the activities of antioxidant enzymes and decreasing the contents of interleukin (IL)-1β and tumor necrosis factor (TNF)-α. Additionally, MFE regulated the expression of miRNA-155 and lipid metabolism-related PPARα protein in rats. Both miR-155 and PPARα play important roles in liver function. The results indicate that MFE has hepatoprotective effects against ALI in rats.

Keywords: Alcoholic liver injury; Gene expression; Lipid metabolism; MicroRNA; Mulberry fruit extract; Oxidative stress; PPARα.

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Antioxidants / pharmacology
  • Fruit / metabolism
  • Humans
  • Lipid Metabolism
  • Lipids
  • Liver / metabolism
  • Liver Diseases, Alcoholic* / drug therapy
  • Liver Diseases, Alcoholic* / genetics
  • Liver Diseases, Alcoholic* / metabolism
  • MicroRNAs* / genetics
  • MicroRNAs* / metabolism
  • Morus* / metabolism
  • Oxidative Stress
  • PPAR alpha / genetics
  • PPAR alpha / metabolism
  • PPAR alpha / pharmacology
  • Rats
  • Tumor Necrosis Factor-alpha / genetics
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • PPAR alpha
  • Antioxidants
  • Tumor Necrosis Factor-alpha
  • MicroRNAs
  • Lipids
  • MIRN155 microRNA, human
  • MIRN155 microRNA, rat