Potassium deficiency stress reduces Rubisco activity in Brassica napus leaves by subcellular acidification decreasing photosynthetic rate

Abstract

Under potassium (K) deficiency photosynthetic carboxylation capacities are limited, affecting the photosynthetic rate of plants. However, it is not clear how ionic K within plants regulates carboxylation capacities. Therefore, the photosynthetic rate (A), ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) characteristics, and cytoplasmic pH of Brassica napus leaves with different K levels were measured to evaluate the effects of K on the carboxylation capacity by regulating subcellular pH. The results showed that biochemical limitation dominates the decrease of A. There was a close positive correlation between A and the Rubisco maximum carboxylation rate (V_cmax), which was closer than that between A and the maximum electron transport rate. The thresholds of leaf K concentrations causing decreased A, V_cmax, and Rubisco initial activity were consistent and close to 1.0% in the hydroponic experiments and 1.2% in the field experiments. K deficiency resulted in decreased Rubisco activity, which reduced carboxylation capacity. Moreover, the Rubisco initial activities in vitro with sufficient K supply or under K deficiency all were significantly reduced when the pH was decreased. The cytoplasmic pH was kept neutral at 7.5 under sufficient K supply, and decreased as the leaf K concentration declined below the threshold. Acidified cytoplasmic environment caused by K deficiency could not maintain the pH balance of the chloroplasts, leading to decreased Rubisco initial activity and photosynthetic capacity.

Keywords: Brassica napus; Photosynthesis; Potassium; Rubisco; Subcellular; pH regulation.