Elevational changes in soil properties shaping fungal community assemblages in terrestrial forest

Abstract

Environmental variables shifted by climate change act as driving factors in determining plant-associated microbial communities in terrestrial ecosystems. However, how elevation-induced changes in soil properties shape the microbial community in forest ecosystems remains less understood. Thus, the Pinus tabuliformis forests at elevations of 1500 m, 1900 m, and 2300 m above sea level were investigated to explore the effect of environmental factors on microbial assemblage. Significant changes in the soil physicochemical properties were found across the investigated elevations, such as soil moisture, temperature, pH, nitrogen (N), and phosphorus (P). Soil enzymatic activities, including soil sucrase, phosphatase, and dehydrogenase, were significantly affected by elevation, and sucrase showed a linear correlation with soil organic matter. Furthermore, the richness of fungal communities in the rhizosphere was decreased as elevation increased, while a humpback pattern was found for roots. Certain core microbiota members, such as Agaricomycetes, Leotiomycetes, and Pezizomycetes, were crucial in maintaining a stable ecological niche in both the root and rhizosphere. We also found that shifting of fungal communities in the rhizosphere were more related to physical properties (e.g., pH, soil moisture, and soil temperature), while changes in root fungal communities along elevation gradient were related mostly to soil nutrients (e.g., soil N and P). Overall, this study demonstrates that the assemblage of the root and rhizosphere fungal communities in P. tabuliformis forest primarily depends on elevation-induced changes in environmental variables and highlights the importance of predicting fungal responses to future climate change.

Keywords: Elevation; Pinus tabuliformis; Root-associated fungi; Soil enzymatic activities; Terrestrial ecosystems.