Transcriptomic Analysis of Liver Tissue of Black Sea Bass (Centropristis striata) Exposed to High Nitrogen Environment

Bingjian Liu; Xun Jin; Kun Zhang; Yifan Liu; Shaowen Wang; Shiyi Chen; Shufei Zhang; Xiaolong Yin

doi:10.3390/genes14071440

Transcriptomic Analysis of Liver Tissue of Black Sea Bass (Centropristis striata) Exposed to High Nitrogen Environment

Genes (Basel). 2023 Jul 13;14(7):1440. doi: 10.3390/genes14071440.

Authors

Bingjian Liu¹, Xun Jin¹, Kun Zhang¹, Yifan Liu¹, Shaowen Wang², Shiyi Chen¹, Shufei Zhang³, Xiaolong Yin⁴

Affiliations

¹ Marine Science and Technology College, Zhejiang Ocean University, Zhoushan 316021, China.
² College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China.
³ Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Guangzhou 510300, China.
⁴ Zhoushan Fisheries Research Institute of Zhejiang Province, Zhoushan 316021, China.

Abstract

The black sea bass, Centropristis striata, is a potential candidate for commercial aquaculture. Due to inadequate removal of nitrogen in its breeding environment, C. striata exhibits increased nitrate concentration, which can cause acute toxicity, including energy metabolism damage and tissue damage. Therefore, RNA-seq technology was applied to characterize genes associated with toxicity tolerance under nitrate stress. The nitrate treatment caused significant changes in a total of 8920 genes, of which 2949 genes were up-regulated and 5971 genes were down-regulated. It was found that significantly enriched GO terms and KEGG were associated with blood microparticles, inhibitors of enzyme activity, and complement and coagulation cascade pathways. Furthermore, through bioinformatics analysis, it was found that these different pathways obtained in GO and KEGG enrichment analysis were mostly related to the immune and inflammatory response of fish. This study expands our understanding of the mechanism of nitrate stress affecting the liver function of C. striata.

Keywords: Centropristis striata; aquaculture; differentially expressed genes; nitrate stress; path enrichment analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bass* / genetics
Bass* / metabolism
Black Sea
Liver / metabolism
Nitrates / metabolism
Transcriptome

Substances

Nitrates

Grants and funding

This research was funded by the Zhejiang Provincial Natural Science Foundation of China (LY22D060001&LY20C190008); National Natural Science Foundation of China (NSFC) (No. 41806156&31702321); Fund of Guangdong Provincial Key Laboratory of Fishery, Ecology, and Environment (FEEL-2021-8); 2019 Zhejiang Province Public Welfare Technology Application Research Project (LGN19C190001); Open Foundation from Marine Sciences in the First-Class Subjects of Zhejiang (No. OFMS010); Student Innovation and Entrepreneurship Training Program Project (202110340031).