Exploring the Antimicrobial, Anticancer, and Apoptosis Inducing Ability of Biofabricated Silver Nanoparticles Using Lagerstroemia speciosa Flower Buds against the Human Osteosarcoma (MG-63) Cell Line via Flow Cytometry

Kariyellappa Nagaraja Shashiraj; Anil Hugar; Raju Suresh Kumar; Muthuraj Rudrappa; Meghashyama Prabhakara Bhat; Abdulrahman I Almansour; Karthikeyan Perumal; Sreenivasa Nayaka

doi:10.3390/bioengineering10070821

Exploring the Antimicrobial, Anticancer, and Apoptosis Inducing Ability of Biofabricated Silver Nanoparticles Using Lagerstroemia speciosa Flower Buds against the Human Osteosarcoma (MG-63) Cell Line via Flow Cytometry

Bioengineering (Basel). 2023 Jul 10;10(7):821. doi: 10.3390/bioengineering10070821.

Authors

Kariyellappa Nagaraja Shashiraj¹, Anil Hugar¹, Raju Suresh Kumar², Muthuraj Rudrappa¹, Meghashyama Prabhakara Bhat¹, Abdulrahman I Almansour², Karthikeyan Perumal³, Sreenivasa Nayaka¹

Affiliations

¹ P.G. Department of Studies in Botany, Karnatak University, Dharwad 580003, Karnataka, India.
² Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
³ Department of Chemistry and Biochemistry, The Ohio State University, 151 W. Woodruff Ave, Columbus, OH 43210, USA.

Abstract

Biosynthesized nano-composites, such as silver nanoparticles (AgNPs), can be engineered to function as smart nano-biomedicine platforms for the detection and management of diverse ailments, such as infectious diseases and cancer. This study determined the eco-friendly fabrication of silver nanoparticles using Lagerstroemia speciosa (L.) Pers. flower buds and their efficacy against antimicrobial and anticancer activities. The UV-Visible spectrum was found at 413 nm showing a typical resonance spectrum for L. speciosa flower bud extract-assisted silver nanoparticles (Ls-AgNPs). Fourier transform infrared analysis revealed the presence of amines, halides, and halogen compounds, which were involved in the reduction and capping agent of AgNP formation. X-ray diffraction analysis revealed the face-centered cubic crystals of NPs. Energy dispersive X-ray verified the weight of 39.80% of silver (Ag), TEM analysis revealed the particles were spherical with a 10.27 to 62.5 nm range, and dynamic light scattering recorded the average particle size around 58.5 nm. Zeta potential showed a significant value at -39.4 mV, and finally, thermo-gravimetric analysis reported higher thermal stability of Ls-AgNPs. Further, the obtained Ls-AgNPs displayed good antimicrobial activity against clinical pathogens. In addition, a dose-dependent decrease in the anticancer activity by MTT assay on the osteosarcoma (MG-63) cell line showed a decrease in the cell viability with increasing in the concentration of Ls-AgNPs with an IC₅₀ value of 37.57 µg/mL. Subsequently, an apoptotic/necrosis study was conducted with the help of Annexin-V/PI assay, and the results indicated a significant rise in early and late apoptosis cell populations. Therefore, green synthesized Ls-AgNPs were found to have potent antimicrobial and anticancer properties making them fascinating choices for future bio-medical implementations.

Keywords: Lagerstroemia speciosa flower bud; MG-63 cancer cell line; MTT assay; apoptosis/necrosis; bio-fabricated synthesis; flow cytometry; silver nanoparticles.

Grants and funding

The project was funded by Researchers Supporting Project number (RSP2023R231) King Saud University, Riyadh, Saudi Arabia/King Saud University