Micro-grinding-based production for sulforaphene-enriched radish seeds extract via facilitating glucosinolates-myrosinase reaction, and evaluation of its anti-adipogenic effects

Tae Kyung Lee; Gihyun Hur; Jong Hun Kim; Jung Han Yoon Park; Hee Yang; Ki Won Lee

doi:10.1016/j.foodchem.2023.136864

Micro-grinding-based production for sulforaphene-enriched radish seeds extract via facilitating glucosinolates-myrosinase reaction, and evaluation of its anti-adipogenic effects

Food Chem. 2023 Dec 15:429:136864. doi: 10.1016/j.foodchem.2023.136864. Epub 2023 Jul 16.

Authors

Tae Kyung Lee¹, Gihyun Hur¹, Jong Hun Kim², Jung Han Yoon Park³, Hee Yang⁴, Ki Won Lee⁵

Affiliations

¹ Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea.
² Department of Food Science and Biotechnology, Sungshin University, Seoul 01133, Republic of Korea; Basic Science Research Institute, Sungshin University, Seoul 01133, Republic of Korea.
³ Bio-MAX Institute, Seoul National University, Seoul 08826, Republic of Korea.
⁴ Department of Food and Nutrition, Kookmin University, Seoul 02707, Republic of Korea. Electronic address: yhee6106@kookmin.ac.kr.
⁵ Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea; Bio-MAX Institute, Seoul National University, Seoul 08826, Republic of Korea; Advanced Institute of Convergence Technology, Seoul National University, Suwon 16229, Republic of Korea; Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea; Institutes of Green Bio Science & Technology, Seoul National University, Pyeongchang 25354, Republic of Korea; Center for Food and Bio convergence, Seoul National University, Seoul 08826, Republic of Korea. Electronic address: kiwon@snu.ac.kr.

PMID: 37506660
DOI: 10.1016/j.foodchem.2023.136864

Abstract

Sulforaphene (SFEN), an isothiocyanate (ITC) abundant in radish (Raphanus sativus) seeds (RS), has many health benefits, including anti-obesity effects. SFEN content is affected by multiple factors during processing, such as glucoraphenin (GLE) (the precursor of SFEN) availability, myrosinase (essential for conversion from GLE to SFEN) activity, and SFEN stability. We examined the physiochemical-properties and anti-adipogenic effects of SFEN-enriched RSE produced by two processes, roasting and micro-grinding. The roasting process lowered SFEN content and myrosinase activity over 50 °C. However, among micro-grinding conditions, smaller particle size (#2 grind, ≈11.31 μm) more effectively increased SFEN content in RS compared to larger particles (#1 grind, ≈ 179.50 μm) by accelerating available GLE and myrosinase release from RS. Grind #2 also effectively inhibited the adipogenesis of 3T3-L1 pre-adipocytes compared to #1. Thus, micro-grinding can be suggested for producing SFEN-enriched RSE with anti-adipogenic activity as a functional material for obesity prevention or treatment.

Keywords: Anti-obesity; Glucoraphenin; Micro-grinding; Myrosinase; Radish seeds; Sulforaphene.

MeSH terms

Adipogenesis
Glucosinolates / pharmacology
Isothiocyanates / pharmacology
Plant Extracts / pharmacology
Raphanus*
Seeds

Substances

sulphoraphene
thioglucosidase
glucoraphenin
Glucosinolates
Isothiocyanates
Plant Extracts