Starvation-inactivated MTOR triggers cell migration via a ULK1-SH3PXD2A/TKS5-MMP14 pathway in ovarian carcinoma

Chiao-Yun Lin; Kai-Yun Wu; Lang-Ming Chi; Yun-Hsin Tang; Huei-Jean Huang; Chyong-Huey Lai; Chi-Neu Tsai; Chia-Lung Tsai

doi:10.1080/15548627.2023.2239633

Starvation-inactivated MTOR triggers cell migration via a ULK1-SH3PXD2A/TKS5-MMP14 pathway in ovarian carcinoma

Autophagy. 2023 Dec;19(12):3151-3168. doi: 10.1080/15548627.2023.2239633. Epub 2023 Jul 28.

Authors

Chiao-Yun Lin¹, Kai-Yun Wu^{1

2}, Lang-Ming Chi³, Yun-Hsin Tang^{1

2}, Huei-Jean Huang^{1

2}, Chyong-Huey Lai^{1

2}, Chi-Neu Tsai^{4

5}, Chia-Lung Tsai⁶

Affiliations

¹ Gynecologic Cancer Research Center, Chang Gung Memorial Hospital, Taoyuan City, Guishan District, Taiwan.
² Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Linkou Medical Center And Chang Gung University, Taoyuan City, Guishan District, Taiwan.
³ Molecular Medicine Research Center, Chang Gung University, Taoyuan City, Guishan District, Taiwan.
⁴ Graduate Institute of Clinical Medical Science, Chang-Gung University, Taoyuan City, Guishan District, Taiwan.
⁵ Department of Surgery, New Taipei Municipal Tucheng Hospital, New Taipei City, Tucheng District, Taiwan.
⁶ Genomic Medicine Core Laboratory, Chang Gung Memorial Hospital, Taoyuan City, Guishan District, Taiwan.

PMID: 37505094
PMCID: PMC10621272 (available on 2024-07-28)
DOI: 10.1080/15548627.2023.2239633

Abstract

AMPK: AMP-activated protein kinase; CHX: cycloheximide; RAD001: everolimus; HBSS: Hanks' balanced salt solution; LC-MS/MS: liquid chromatography-mass spectrometry/mass spectrometry; MMP14: matrix metallopeptidase 14; MTOR: mechanistic target of rapamycin kinase; MAPK: mitogen-activated protein kinase; RB1CC1/FIP200: RB1 inducible coiled-coil 1; PtdIns3P: phosphatidylinositol-3-phosphate; PX: phox homology; SH3: Src homology 3; SH3PXD2A/TKS5: SH3 and PX domains 2A; SH3PXD2A-[6A]: S112A S142A S146A S147A S175A S348A mutant; ULK1: unc-51 like autophagy activating kinase 1.

Keywords: MMP14; SH3PXD2A; ULK1; cellular migration; ovarian cancer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

AMP-Activated Protein Kinases / metabolism
Adaptor Proteins, Vesicular Transport
Autophagy*
Autophagy-Related Protein-1 Homolog / metabolism
Cell Movement
Chromatography, Liquid
Female
Humans
Intracellular Signaling Peptides and Proteins
Matrix Metalloproteinase 14
Ovarian Neoplasms*
TOR Serine-Threonine Kinases / metabolism
Tandem Mass Spectrometry

Substances

Matrix Metalloproteinase 14
AMP-Activated Protein Kinases
TOR Serine-Threonine Kinases
Adaptor Proteins, Vesicular Transport
Autophagy-Related Protein-1 Homolog
MMP14 protein, human
MTOR protein, human
SH3PXD2A protein, human
ULK1 protein, human
Intracellular Signaling Peptides and Proteins

Grants and funding

This study was financially supported by grants from the National Science and Technology Council, Taiwan (111-2314-B-182A-035-MY3 to CLT; 110-2314-B-182-033 to CNT; 111-2314-B-182A-088 to CYL) and Chang Gung Medical Research Foundation, Taiwan (CMRPG3L1431, CMRPG3M0801, and CMRPG3M1291 to CLT, CMRPG3M0761 to CYL).