Biomimetic, biodegradable and osteoinductive treated dentin matrix/α-calcium sulphate hemihydrate composite material for bone tissue engineering

Runying Guo; Rui Zhang; Sirui Liu; Yanyu Yang; Wenhang Dong; Meiyue Wang; Hongyan Mi; Mengzhe Liu; Jingjing Sun; Xue Zhang; Yimeng Su; Yiming Liu; Di Huang; Rui Li

doi:10.1093/rb/rbad061

Biomimetic, biodegradable and osteoinductive treated dentin matrix/α-calcium sulphate hemihydrate composite material for bone tissue engineering

Regen Biomater. 2023 Jun 19:10:rbad061. doi: 10.1093/rb/rbad061. eCollection 2023.

Authors

Runying Guo^{1

2}, Rui Zhang³, Sirui Liu¹, Yanyu Yang⁴, Wenhang Dong¹, Meiyue Wang¹, Hongyan Mi¹, Mengzhe Liu¹, Jingjing Sun¹, Xue Zhang¹, Yimeng Su⁵, Yiming Liu¹, Di Huang⁵, Rui Li¹

Affiliations

¹ Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, PR China.
² Department of Stomatology, The First Affiliated Hospital of Nanchang University, Nanchang 330000, PR China.
³ Oral and Maxillofacial Department, Zhengzhou Stomatology Hospital, Zhengzhou 450000, PR China.
⁴ College of Materials Science and Engineering, Zhengzhou University, Zhengzhou 450000, PR China.
⁵ Research Center for Nano-biomaterials and Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China.

Abstract

It is still a huge challenge for bone regenerative biomaterial to balance its mechanical, biological and biodegradable properties. In the present study, a new composite material including treated dentin matrix (TDM) and α-calcium sulphate hemihydrate (α-CSH) was prepared. The optimal composition ratio between TDM and α-CSH was explored. The results indicate that both components were physically mixed and structurally stable. Its compressive strength reaches up to 5.027 ± 0.035 MPa for 50%TDM/α-CSH group, similar to human cancellous bone tissues. Biological experiments results show that TDM/α-CSH composite exhibits excellent biocompatibility and the expression of osteogenic related genes and proteins (ALP, RUNX2, OPN) is significantly increased. In vivo experiments suggest that the addition of TDM for each group (10%, 30%, 50%) effectively promotes cell proliferation and osteomalacia. In addition, 50% of the TDM/α-CSH combination displays optimal osteoconductivity. The novel TDM/α-CSH composite is a good candidate for certain applications in bone tissue engineering.

Keywords: bone defect; osteogenesis; treated dentin matrix; α-calcium sulphate hemihydrate.