Research Note: Identification of breeding-related candidate genes in Tianjin-monkey chickens by transcriptome analysis

Shuli Xia; Xianghua Zhao; Haitao Yu; Guohui Li

doi:10.1016/j.psj.2023.102928

Research Note: Identification of breeding-related candidate genes in Tianjin-monkey chickens by transcriptome analysis

Poult Sci. 2023 Oct;102(10):102928. doi: 10.1016/j.psj.2023.102928. Epub 2023 Jul 9.

Authors

Shuli Xia¹, Xianghua Zhao¹, Haitao Yu¹, Guohui Li²

Affiliations

¹ Institute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin, 300381, China; Tianjin Key Laboratory of Animal Molecular Breeding and Biotechnology, Tianjin, 300381, China; Tianjin Engineering Research Center of Animal Healthy Farming, Tianjin, 300381, China.
² Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, 225125, China. Electronic address: sahui2008@163.com.

Abstract

Tianjin-monkey Chicken is a locally bred dual-purpose naked neck poultry with high tolerance to heat stress and poor reproductive ability. We aim to explore breeding-related genes to promote its growth, reproduction, meat, and egg performances. In this study, purebred, crossbred neck-naked and crossbred neck-feathered Tianjin-monkey Chickens (male = 5 and female = 5 in each group) were sampled for transcriptome analysis. Differential gene expression analysis was performed to identify candidate genes based on mRNA expression profiles. Functional enrichment analyses including GO, KEGG and GSEA analysis were conducted. Forty-five candidate breeding-related genes were identified, which were significantly enriched in 5 KEGG pathways and 37 GO terms. Some of the candidate genes were considered to be valuable in guiding breeding in the future, including SPRY3, CPXM2, FST, HDDC2, TLR1B, CYBB, and EHHADH.

Keywords: Tianjin-monkey Chicken; breeding; transcriptome.

MeSH terms

Animals
Chickens* / genetics
Feathers
Female
Gene Expression Profiling* / veterinary
Male
Meat
Reproduction / genetics
Transcriptome