Inhibiting mechanism of Alpiniae oxyphyllae fructus polysaccharide 3 against the replication of porcine epidemic diarrhea virus

Haowen Wu; Qiyuan Luo; Yijing Chen; Suya Liao; Huricha Chen; Yun Chen; Yao Qin

doi:10.1016/j.virol.2023.109848

Inhibiting mechanism of Alpiniae oxyphyllae fructus polysaccharide 3 against the replication of porcine epidemic diarrhea virus

Virology. 2023 Oct:587:109848. doi: 10.1016/j.virol.2023.109848. Epub 2023 Jul 23.

Authors

Haowen Wu¹, Qiyuan Luo¹, Yijing Chen¹, Suya Liao¹, Huricha Chen¹, Yun Chen², Yao Qin³

Affiliations

¹ Institute of Traditional South Chinese Veterinary Pharmacology, School of Animal Science and Technology of Hainan University, Haikou, 570228, PR China.
² Institute of Traditional South Chinese Veterinary Pharmacology, School of Animal Science and Technology of Hainan University, Haikou, 570228, PR China. Electronic address: ychen@hainanu.edu.cn.
³ Institute of Traditional South Chinese Veterinary Pharmacology, School of Animal Science and Technology of Hainan University, Haikou, 570228, PR China. Electronic address: qinyao@hainanu.edu.cn.

PMID: 37499528
DOI: 10.1016/j.virol.2023.109848

Abstract

Porcine epidemic diarrhea virus (PEDV) causes diarrhea, vomiting, and death in piglets. Our previous study has revealed the anti-PEDV activity of Alpiniae oxyphyllae fructus polysaccharide 3 (AOFP3). However, it is still unknown whether AOFP3 can inhibit the replication of PEDV. Therefore, the effect of AOFP3 on PEDV replication was investigated in the present study, along with analysis of viral RdRp activity and expression of hnRNP A1 by RNA polymerase activity assay in vitro, RIP assay, and Western blotting. The results showed that both the PEDV gene and protein levels in IPEC-J2 cells decreased with AOFP3 treatment. In addition, AOFP3 significantly reduced PEDV's replication by down-regulating the activity of PEDV RdRp and reducing the expression of hnRNP A1, whereas only the bind of RdRp to PEDV 3'UTR was inhibited in AOFP3 treated cells.

Keywords: Alpiniae oxyphyllae fructus polysaccharide 3; Heterogeneous nuclear ribonucleoprotein A1; Porcine epidemic diarrhea virus; RNA-Dependent RNA polymerase; Viral replication.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Coronavirus Infections*
Heterogeneous Nuclear Ribonucleoprotein A1
Porcine epidemic diarrhea virus* / physiology
RNA-Dependent RNA Polymerase
Swine
Swine Diseases* / drug therapy
Virus Replication

Substances

Heterogeneous Nuclear Ribonucleoprotein A1
RNA-Dependent RNA Polymerase