Lysophospholipids transport across blood-brain barrier in an in vitro reconstruction model

Tamotsu Tsukahara; Masanori Sasaki; Hisao Haniu; Yoshikazu Matsuda

doi:10.1016/j.bbrc.2023.07.044

Lysophospholipids transport across blood-brain barrier in an in vitro reconstruction model

Biochem Biophys Res Commun. 2023 Oct 8:676:91-96. doi: 10.1016/j.bbrc.2023.07.044. Epub 2023 Jul 22.

Authors

Tamotsu Tsukahara¹, Masanori Sasaki², Hisao Haniu³, Yoshikazu Matsuda⁴

Affiliations

¹ Department of Pharmacology and Therapeutic Innovation, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. Electronic address: ttamotsu@nagasaki-u.ac.jp.
² Department of Pharmacology and Therapeutic Innovation, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
³ Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Matsumoto, 390-8621, Japan.
⁴ Division of Clinical Pharmacology and Pharmaceutics, Nihon Pharmaceutical University, Ina, 362-0806, Japan.

PMID: 37499369
DOI: 10.1016/j.bbrc.2023.07.044

Abstract

This study builds on our previous study, which highlighted the need for further research on the potential use of lysophospholipid (LPL) supplementation to prevent chronic and age-related diseases. We aimed to evaluate the transmembrane transport of LPL across rat and monkey blood-brain barrier (BBB) models. An in vitro monkey BBB model is required to elucidate the differences between rat and primate BBB-related data and to measure the permeability of LPLs being researched in relation to the human BBB. Based on our previous experiment, porcine liver decomposition product-derived phospholipids (PEL) strongly inhibit α-synuclein (α-Syn) aggregation. We have identified several candidates potentially relevant for the inhibition of α-Syn aggregation, such as LPC18:1, LPE18:1, and LPI18:0; however, the BBB permeability of these LPLs remains unclear. In the present study, we assessed the ability of these LPLs to pass through the in vitro rat and monkey BBB models. LPC18:1 showed high BBB permeability, LPI18:0 showed medium permeability, and the BBB permeation of LPE18:1 was negligible. Our results suggest that LPC18:1 and LPI18:0 are functional food factors that can cross the BBB.

Keywords: Blood-brain barrier; Lysophospholipid; Transmembrane transport; α-Syn aggregation.