Biomimetic hydrogel derived from decellularized dermal matrix facilitates skin wounds healing

Yaling Yu; Huimin Xiao; Guoke Tang; Hongshu Wang; Junjie Shen; Yi Sun; Shuaiqun Wang; Wei Kong; Yimin Chai; Xuanzhe Liu; Xing Wang; Gen Wen

doi:10.1016/j.mtbio.2023.100725

Biomimetic hydrogel derived from decellularized dermal matrix facilitates skin wounds healing

Mater Today Bio. 2023 Jul 7:21:100725. doi: 10.1016/j.mtbio.2023.100725. eCollection 2023 Aug.

Authors

Yaling Yu^{1

2}, Huimin Xiao^{1

3}, Guoke Tang⁴, Hongshu Wang¹, Junjie Shen¹, Yi Sun¹, Shuaiqun Wang⁵, Wei Kong⁵, Yimin Chai¹, Xuanzhe Liu¹, Xing Wang^{6

7}, Gen Wen¹

Affiliations

¹ Department of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200233, China.
² Institute of Microsurgery on Extremities, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200233, China.
³ College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
⁴ Department of Orthopedics, Shanghai General Hospital, Shanghai Jiaotong University, Shanghai, 200080, China.
⁵ College of Information Engineering, Shanghai Maritime University, Shanghai, 201306, China.
⁶ Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
⁷ University of Chinese Academy of Sciences, Beijing, 100049, China.

Abstract

Cutaneous wound healing affecting millions of people worldwide represents an unsolvable clinical issue that is frequently challenged by scar formation with dramatical pain, impaired mobility and disfigurement. Herein, we prepared a kind of light-sensitive decellularized dermal extracellular matrix-derived hydrogel with fast gelling performance, biomimetic porous microstructure and abundant bioactive functions. On account of its excellent cell biocompatibility, this ECM-derived hydrogel could induce a marked cellular infiltration and enhance the tube formation of HUVECs. In vivo experiments based upon excisional wound splinting model showed that the hydrogel prominently imparted skin wound healing, as evidenced by notably increased skin appendages and well-organized collagen expression, coupled with significantly enhanced angiogenesis. Moreover, the skin regeneration mediated by this bioactive hydrogel was promoted by an accelerated M1-to-M2 macrophage phenotype transition. Consequently, the decellularized dermal matrix-derived bioactive hydrogel orchestrates the entire skin healing microenvironment to promote wound healing and will be of high value in treatment of cutaneous wound healing. As such, this biomimetic ddECMMA hydrogel provides a promising versatile opinion for the clinical translation.

Keywords: Decellularization; Dermal tissue; Immunomodulation; Photo-responsibility; Wound healing.