Biopolymer collagen-chitosan scaffold containing Aloe vera for chondrogenic efficacy on cartilage tissue engineering

Panneerselvam Jithendra; Jamal Moideen Muthu Mohamed; Dinesh Annamalai; Rasha Hamed Al-Serwi; Ateya Megahed Ibrahim; Mohamed El-Sherbiny; Abraham Merlin Rajam; Mamdouh Eldesoqui; Nimer Mansour

doi:10.1016/j.ijbiomac.2023.125948

Biopolymer collagen-chitosan scaffold containing Aloe vera for chondrogenic efficacy on cartilage tissue engineering

Int J Biol Macromol. 2023 Sep 1:248:125948. doi: 10.1016/j.ijbiomac.2023.125948. Epub 2023 Jul 22.

Authors

Panneerselvam Jithendra¹, Jamal Moideen Muthu Mohamed², Dinesh Annamalai³, Rasha Hamed Al-Serwi⁴, Ateya Megahed Ibrahim⁵, Mohamed El-Sherbiny⁶, Abraham Merlin Rajam⁷, Mamdouh Eldesoqui⁸, Nimer Mansour⁹

Affiliations

¹ Department of Biotechnology, CSIR-Central Leather Research Institute, Chennai 600020, India; Department of Pharmaceutical Technology, School of Pharmacy, International Medical University, Kuala Lumpur, Malaysia. Electronic address: jithendra@imu.edu.my.
² Vaasudhara College of Pharmacy, Sante Circle, Chintamani Road, Hoskote, 562114, Karnataka, India.
³ Centre for Academic and Research Excellence, CSIR-Central Leather Research Institute, Chennai 600020, India.
⁴ Department of Basic Dental Sciences, College of Dentistry, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia. Electronic address: rhalserwi@pnu.edu.sa.
⁵ Department of Nursing, College of Applied Medical Sciences, Prince Sattam bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia; Department of Family and Community Health Nursing, Faculty of Nursing, Port Said University, Egypt. Electronic address: a.eleglany@psau.edu.sa.
⁶ Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, P.O. Box 71666, Riyadh 11597, Saudi Arabia; Department of Anatomy, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt. Electronic address: msharbini@mcst.edu.sa.
⁷ Medical Research center, Women's wellness Research Center, Hamad Medical Corporation, Doha, Qatar.
⁸ Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, P.O. Box 71666, Riyadh 11597, Saudi Arabia; Department of Anatomy, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt. Electronic address: mamrah@mcst.edu.sa.
⁹ Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, P.O. Box 71666, Riyadh 11597, Saudi Arabia. Electronic address: nmansour@mcst.edu.sa.

PMID: 37482169
DOI: 10.1016/j.ijbiomac.2023.125948

Abstract

The chondrogenic efficacy of aloe vera blended collagen-chitosan (COL-CS-AV) porous scaffold was investigated using articular chondrocytes in a standard condition. Cytocompatibility was analyzed using fluorescent dyes (calcein AM/ethidium bromide) and the viable cells were quantified by MTT assay. Glycosaminoglycan (GAG) content of ECM was estimated by using 1, 9-Dimethyl methylene Blue (DMMB). The total RNA content was quantified and the cartilage specific genes (col2a1, Acan) were amplified by reverse transcription-PCR from the cell lysate of the scaffolds. Histological examination was made using Haematoxylin and Eosin (H&E), safranin-O, masson's trichrome, alcian blue, and alizarin red to stain the specific component of ECM secreted on the construct. The cartilage specific collagen type II was estimated by immunohistochemistry using monoclonal type II collagen antibody. The results of these studies proved that COL-CS-AV scaffold has more chondrogenic efficacy than COL-CS, thus the aloe vera blend COL-CS-AV scaffold might be used as suitable candidate for cartilage tissue engineering.

Keywords: Aloe vera; Cartilage tissue engineering; Chitosan; Chondrocyte; Collagen; Scaffold.

MeSH terms

Aloe*
Cartilage / metabolism
Cells, Cultured
Chitosan* / chemistry
Chondrocytes / metabolism
Collagen / chemistry
Collagen Type II / metabolism
Tissue Engineering / methods
Tissue Scaffolds / chemistry

Substances

Chitosan
Collagen
Collagen Type II