A simple, low cost and fast sample preparation method for fluorine determination by ISE in leguminous seeds and oilseeds

Abstract

This work presents the first report of the application of microwave-induced combustion in disposable vessels (MIC-DV) as a simple and fast sample preparation step for fluoride determination by ion-selective electrode (ISE) in leguminous seeds and oilseeds samples. Several experimental conditions of MIC-DV were evaluated, such as sample mass, absorbing solution, volume of combustion igniter, purging time with oxygen, and the use of successive combustion cycles. The accuracy of MIC-DV/ISE method was evaluated by comparing the results obtained by microwave-induced combustion and determination by ion chromatography, with agreements ranged from 94 to 106%. The method allowed to achieve relatively low limits of quantification (8.3 to 16 µg g^-1) comparing to AOAC method (40 µg g^-1), besides the use of small amounts of reagents, less handling of digests, making the method greener and with portable features comparing to other methods.

Keywords: Fluoride determination; ISE determination; Leguminous seed analysis; MIC-DV; Oilseed analysis; Sample preparation.