Hemostatic patch with ultra-strengthened mechanical properties for efficient adhesion to wet surfaces

Yuting Zheng; Kaavian Shariati; Mahsa Ghovvati; Steven Vo; Nolan Origer; Taichiro Imahori; Naoki Kaneko; Nasim Annabi

doi:10.1016/j.biomaterials.2023.122240

Hemostatic patch with ultra-strengthened mechanical properties for efficient adhesion to wet surfaces

Biomaterials. 2023 Oct:301:122240. doi: 10.1016/j.biomaterials.2023.122240. Epub 2023 Jul 12.

Authors

Yuting Zheng¹, Kaavian Shariati², Mahsa Ghovvati³, Steven Vo¹, Nolan Origer¹, Taichiro Imahori⁴, Naoki Kaneko⁴, Nasim Annabi⁵

Affiliations

¹ Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA.
² David Geffen School of Medicine, University of California - Los Angeles, Los Angeles, CA 90095, USA.
³ Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA; Division of Interventional Neuroradiology, Department of Radiological Sciences, David Geffen School of Medicine, University of California - Los Angeles, Los Angeles, CA 90095, USA.
⁴ Division of Interventional Neuroradiology, Department of Radiological Sciences, David Geffen School of Medicine, University of California - Los Angeles, Los Angeles, CA 90095, USA.
⁵ Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90095, United States. Electronic address: nannabi@ucla.edu.

PMID: 37480758
DOI: 10.1016/j.biomaterials.2023.122240

Abstract

Controlling traumatic bleeding from damaged internal organs while effectively sealing the wound is critical for saving the lives of patients. Existing bioadhesives suffer from blood incompatibility, insufficient adhesion to wet surfaces, weak mechanical properties, and complex application procedures. Here, we engineered a ready-to-use hemostatic bioadhesive with ultra-strengthened mechanical properties and fatigue resistance, robust adhesion to wet tissues within a few seconds of gentle pressing, deformability to accommodate physiological function and action, and the ability to stop bleeding efficiently. The engineered hydrogel, which demonstrated high elasticity (>900%) and toughness (>4600 kJ/m³), was formed by fine-tuning a series of molecular interactions and crosslinking mechanisms involving N-hydroxysuccinimide (NHS) conjugated alginate (Alg-NHS), poly (ethylene glycol) diacrylate (PEGDA), tannic acid (TA), and Fe³⁺ ions. Dual adhesive moieties including mussel-inspired pyrogallol/catechol and NHS synergistically enhanced wet tissue adhesion (>400 kPa in a wound closure test). In conjunction with physical sealing, the high affinity of TA/Fe³⁺ for blood could further augment hemostasis. The engineered bioadhesive demonstrated excellent in vitro and in vivo biocompatibility as well as improved hemostatic efficacy as compared to commercial Surgicel®. Overall, the hydrogel design strategy described herein holds great promise for overcoming existing obstacles impeding clinical translation of engineered hemostatic bioadhesives.

Keywords: Bioadhesives; Hemostatic materials; Molecular design; Tough hydrogels.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Hemostasis
Hemostatics* / pharmacology
Humans
Hydrogels
Physical Phenomena
Tissue Adhesions

Substances

Hemostatics
Hydrogels

Abstract

Publication types

MeSH terms

Substances

Grants and funding