Formulation and characterization of choline oleate-based micelles for co-delivery of luteolin, naringenin, and quercetin

Islam Md Shimul; Rahman Md Moshikur; Fahmida Habib Nabila; Muhammad Moniruzzaman; Masahiro Goto

doi:10.1016/j.foodchem.2023.136911

Formulation and characterization of choline oleate-based micelles for co-delivery of luteolin, naringenin, and quercetin

Food Chem. 2023 Dec 15:429:136911. doi: 10.1016/j.foodchem.2023.136911. Epub 2023 Jul 17.

Authors

Islam Md Shimul¹, Rahman Md Moshikur², Fahmida Habib Nabila², Muhammad Moniruzzaman³, Masahiro Goto⁴

Affiliations

¹ Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan; Department of Nutrition and Food Technology, Jashore University of Science and Technology, Jashore 7408, Bangladesh.
² Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
³ Department of Chemical Engineering, Universiti Teknologi PETRONAS, 32610 Seri Iskandar, Perak, Malaysia.
⁴ Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan; Division of Biotechnology, Center for Future Chemistry, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan; Advanced Transdermal Drug Delivery System Center, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address: m-goto@mail.cstm.kyushu-u.ac.jp.

PMID: 37478610
DOI: 10.1016/j.foodchem.2023.136911

Abstract

Flavonoids have diverse beneficial roles that potentiate their application as nutraceutical agents in nutritional supplements and as natural antimicrobial agents in food preservation. To address poor solubility and bioactivity issues, we developed water-soluble micellar formulations loaded with single and multiple flavonoids using the biocompatible surface-active ionic liquid choline oleate. The food preservation performance was investigated using luteolin, naringenin, and quercetin as model bioactive compounds. The micellar formulations formed spherical micelles with particle sizes of <150 nm and exhibited high aqueous solubility (>5.15 mg/mL). Co-delivery of multiple flavonoids (luteolin, naringenin, and quercetin in LNQ-MF) resulted in 84.85% antioxidant activity at 100 μg/mL. The effects on Staphylococcus aureus and Salmonella enterica were synergistic with fractional inhibitory concentration indices of 0.87 and 0.71, respectively. LNQ-MF hindered the growth of S. aureus in milk (0.83-0.89 log scale) compared to the control. Co-delivered encapsulated flavonoids are a promising alternative to chemical preservatives.

Keywords: Bioactive compound; Co-delivery; Ionic liquid; Micelle formulation; Milk preservation; Synergism.

MeSH terms

Flavonoids / chemistry
Luteolin / pharmacology
Micelles*
Oleic Acid
Quercetin* / chemistry
Staphylococcus aureus

Substances

Quercetin
naringenin
Micelles
Oleic Acid
Luteolin
Flavonoids