The Search for Single-Domain Antibodies Interacting with the Receptor-Binding Domain of SARS-CoV-2 Surface Protein

Bull Exp Biol Med. 2023 Jun;175(2):225-228. doi: 10.1007/s10517-023-05839-6. Epub 2023 Jul 19.

Abstract

We performed a search for nanoantibodies that specifically interact with the receptor-binding domain (RBD) of the SARS-CoV-2 surface protein. The specificity of single-domain antibodies from the blood sera of a llama immunized with RBD of SARS-CoV-2 surface protein S (variant B.1.1.7 (Alpha)) was analyzed by ELISA. Recombinant trimers of the SARS-CoV-2 spike protein were used as antigens. In this work, a set of single-domain antibodies was obtained that specifically bind to the RBD of the SARS-CoV-2 virus.

Keywords: SARS-CoV-2; affinity selection; bacteriophage; phage library; single-domain antibodies.

MeSH terms

  • Antibodies, Neutralizing
  • Antibodies, Viral
  • COVID-19*
  • Humans
  • Membrane Proteins
  • SARS-CoV-2
  • Single-Domain Antibodies* / genetics

Substances

  • spike protein, SARS-CoV-2
  • Single-Domain Antibodies
  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Membrane Proteins

Supplementary concepts

  • SARS-CoV-2 variants