Facile pretreatment strategies to biotransform Kans grass into nanocatalyst, cellulolytic enzymes, and fermentable sugars towards sustainable biorefinery applications

Preeti Singh; Neha Srivastava; Akbar Mohammad; Basant Lal; Rajeev Singh; Asad Syed; Abdallah M Elgorban; Meenakshi Verma; P K Mishra; Vijai Kumar Gupta

doi:10.1016/j.biortech.2023.129491

Facile pretreatment strategies to biotransform Kans grass into nanocatalyst, cellulolytic enzymes, and fermentable sugars towards sustainable biorefinery applications

Bioresour Technol. 2023 Oct:386:129491. doi: 10.1016/j.biortech.2023.129491. Epub 2023 Jul 16.

Authors

Affiliations

¹ Technology Business Incubator, Indian Institute of Technology (BHU) Varanasi, Varanasi, Uttar Pradesh 221005, India.
² Department of Chemical Engineering & Technology, Indian Institute of Technology (BHU) Varanasi, Varanasi, Uttar Pradesh 221005, India.
³ School of Chemical Engineering, Yeungnam University, Gyeongsan 38541, Republic of Korea.
⁴ Department of Chemistry, Institute of Applied Sciences and Humanities, GLA University, Mathura, Uttar Pradesh 281406, India.
⁵ Department of Environmental Science, Jamia Millia Islamia, A Central University, New Delhi 110025, India.
⁶ Department of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia.
⁷ University Centre for Research & Development, Department of Chemistry, Chandigarh University, Gharuan, Mohali 140413, India.
⁸ Biorefining and Advance Material Research Centre, SRUC, Barony Campus, Parkgate, Dumfries DG1 3NE, United Kingdom; Center for Safe and Improved Food, Scotland's Rural College (SRUC), Kings Buildings, West Mains Road, Edinburgh EH9 3JG, United Kingdom. Electronic address: vijai.gupta@sruc.ac.uk.

PMID: 37463616
DOI: 10.1016/j.biortech.2023.129491

Abstract

The present investigation is targeted towards the facile fabrication of a carbon-based nanocatalyst (CNCs) using Kans grass biomass (KGB) and its sustainable application in microbial cellulase enhancement for the alleviation of enzymatic hydrolysis for sugar production. Different pretreatments, including physical, KGB extract-mediated treatment, followed by KOH pretreatment, have been applied to produce CNCs using KGB. The presence of CNCs influences the pretreatment of KGB substrate, fungal cellulase production, stability, and sugar recovery in the enzymatic hydrolysis of KGB. Using 1.0% CNCs pretreated KGB-based solid-state fermentation, 33 U/gds FPA and 126 U/gds BGL were obtained at 72 h, followed by 107 U/gds EG at 48 h in the presence of 0.5% CNCs. Further, 42 °C has been identified as the optimum temperature for cellulase production, while the enzyme showed thermal stability at 50 °C up to 20 h and produced 38.4 g/L sugar in 24 h through enzymatic hydrolysis of KGB.

Keywords: Cellulolytic enzymes; Lignocellulosic biomass; Nanocatalyst; Pretreatment process; Reducing sugars.

MeSH terms

Biomass
Carbohydrates
Cellulase* / metabolism
Fermentation
Hydrolysis
Poaceae* / metabolism
Sugars
Temperature

Substances

Sugars
Carbohydrates
Cellulase