Targeted DNA N6-methyladenine editing by dCas9 fused to METTL4 in the lepidopteran model insect Bombyx mori

Feng Chen; Hao Guo; Weiqun Lan; Mingyi Zhou; Wenjing Geng; Guanwang Shen; Ping Lin; Qingyou Xia; Ping Zhao; Zhiqing Li

doi:10.1111/1744-7917.13222

Targeted DNA N⁶-methyladenine editing by dCas9 fused to METTL4 in the lepidopteran model insect Bombyx mori

Insect Sci. 2024 Apr;31(2):646-650. doi: 10.1111/1744-7917.13222. Epub 2023 Jul 17.

Authors

Feng Chen^#^{1

2}, Hao Guo^#^{1

2}, Weiqun Lan^{1

2}, Mingyi Zhou^{1

2}, Wenjing Geng^{1

2}, Guanwang Shen^{1

2}, Ping Lin^{1

2}, Qingyou Xia^{1

2}, Ping Zhao^{1

2}, Zhiqing Li^{1

2}

Affiliations

¹ Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Biological Science Research Center, Southwest University, Chongqing, China.
² Chongqing Key Laboratory of Sericultural Science, Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Chongqing, China.

^# Contributed equally.

PMID: 37461250
DOI: 10.1111/1744-7917.13222

Abstract

We have established a novel CRISPR-dCas9-METTL4 epigenome editing tool that can methylate target regions to achieve site-specific DNA 6mA methylation in both hypermethylated and hypomethylated genes. Targeted methylation on genes by dCas9-METTL4 results in misexpression, allowing for the functional investigation of target genes of interest in silkworm.

Publication types

Letter

MeSH terms

Adenine*
Animals
Bombyx* / genetics
CRISPR-Cas Systems
DNA / genetics
DNA Methylation

Substances

6-methyladenine
DNA
Adenine

Abstract

Publication types

MeSH terms

Substances

Grants and funding