Lipidomic profiling study on neurobehavior toxicity in zebrafish treated with aflatoxin B1

Zishuang Wang; Xiabing Li; Tiancai Wang; Guangqin Liao; Jingyi Gu; Ruyan Hou; Jing Qiu

doi:10.1016/j.scitotenv.2023.165553

Lipidomic profiling study on neurobehavior toxicity in zebrafish treated with aflatoxin B1

Sci Total Environ. 2023 Nov 10:898:165553. doi: 10.1016/j.scitotenv.2023.165553. Epub 2023 Jul 17.

Authors

Zishuang Wang¹, Xiabing Li², Tiancai Wang², Guangqin Liao², Jingyi Gu², Ruyan Hou³, Jing Qiu⁴

Affiliations

¹ State Key Laboratory of Tea Plant Biology and Utilization, College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, China; Key Laboratory of Argo-Product Quality and Safety of Ministry of Agriculture, Institute of Quality Standards and Testing Technology for Argo-Products, Chinese Academy of Agricultural Sciences, No. 12 Zhong-guan-cun South Street, Haidian District, Beijing 100081, China.
² Key Laboratory of Argo-Product Quality and Safety of Ministry of Agriculture, Institute of Quality Standards and Testing Technology for Argo-Products, Chinese Academy of Agricultural Sciences, No. 12 Zhong-guan-cun South Street, Haidian District, Beijing 100081, China.
³ State Key Laboratory of Tea Plant Biology and Utilization, College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, China. Electronic address: hry@ahau.edu.cn.
⁴ Key Laboratory of Argo-Product Quality and Safety of Ministry of Agriculture, Institute of Quality Standards and Testing Technology for Argo-Products, Chinese Academy of Agricultural Sciences, No. 12 Zhong-guan-cun South Street, Haidian District, Beijing 100081, China. Electronic address: qiujing@caas.cn.

PMID: 37459993
DOI: 10.1016/j.scitotenv.2023.165553

Abstract

Mycotoxin aflatoxin B1 (AFB1) has been proven to cause neurotoxicity, but its potential interference with the normal function of brain tissue is not fully defined. As the indispensable role of lipids in maintaining the normal function of brain tissue, the aim of this study is to clarify the effect of AFB1 short-term (7 days) exposure on brain tissue from the perspective of lipid metabolism. In this study, zebrafish were exposed to two concentrations (5, 20 μg/L). Through quantitative analysis of AFB1, the detection of AFB1 in zebrafish brain tissue was discovered for the first time, combined with the changes in zebrafish neurobehavior, the occurrence of brain injury was deduced. Subsequently, 1734 lipids in zebrafish brain tissue were mapped using ion mobility time-of-flight mass spectrometry (UPLC-QTOF-IMS-MS), which has great advantages in lipid detection. Comparative analysis of the abnormal lipid metabolism in zebrafish brain revealed 114 significantly changed lipids, mainly involving two pathways of sphingolipid metabolism and fatty acid degradation. This study discovered the detection of AFB1 in the brain and revealed a potential link between AFB1-induced behavioral abnormalities and lipid metabolism disorders in brain tissue, providing reliable evidence for elucidating the neurotoxicity of AFB1.

Keywords: Aflatoxin B1; Behavior analysis; Brain tissue; Lipidomics; Zebrafish.

MeSH terms

Aflatoxin B1 / toxicity
Animals
Lipidomics
Lipids
Mycotoxins* / metabolism
Zebrafish* / metabolism

Substances

Aflatoxin B1
Mycotoxins
Lipids