Probiotics formulation and cancer nanovaccines show synergistic effect in immunotherapy and prevention of colon cancer

Xiangxiang Xu; Meng Zhang; Xiaoyan Liu; Mingze Chai; Lu Diao; Lin Ma; Shuang Nie; Minghao Xu; Yipeng Wang; Fengfeng Mo; Mi Liu

doi:10.1016/j.isci.2023.107167

Probiotics formulation and cancer nanovaccines show synergistic effect in immunotherapy and prevention of colon cancer

iScience. 2023 Jun 19;26(7):107167. doi: 10.1016/j.isci.2023.107167. eCollection 2023 Jul 21.

Authors

Xiangxiang Xu^{1

2

3}, Meng Zhang¹, Xiaoyan Liu¹, Mingze Chai¹, Lu Diao^{1

2

3}, Lin Ma², Shuang Nie⁴, Minghao Xu^{1

3}, Yipeng Wang¹, Fengfeng Mo⁴, Mi Liu^{1

2

3}

Affiliations

¹ College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215123, People's Republic of China.
² Kunshan Hospital of Traditional Chinese Medicine, Kunshan, Jiangsu 215300, People's Republic of China.
³ Suzhou Ersheng Biopharmaceutical Co., Ltd, Suzhou 215123, People's Republic of China.
⁴ Department of Nutrition and Food Hygiene, Faculty of Naval Medicine, Naval Medical University, 800 Xiangyin Rd, Shanghai 200433, People's Republic of China.

Abstract

Probiotics play essential roles in immune modulation. Combining probiotics with cancer vaccines potentially can achieve a synergistic effect. To maximize the efficacy of probiotics, proper probiotics formulation is necessary. Herein, Lactobacillus rhamnosus and Bifidobacterium longum are coated with lipid membrane to achieve the goal of losing less activity and bettering colonization in colon. In the subcutaneous transplanted colon cancer mouse model, probiotics formulation showed potent preventive and therapeutic efficacy, and the efficacy could be further improved by combining with cancer nanovaccines. Probiotics formulation can perform as immune adjuvants to enhance the innate immune response or as in-situ cancer vaccines. In the study of preventing chemical-induced orthotopic colon cancer model, probiotics formulation alone efficiently reduced tumor number in colon and the efficacy is improved by combining with cancer nanovaccines. All in all, the studies demonstrated that probiotics formulation can assist to maximize the efficacy of cancer nanovaccines.

Keywords: Biological sciences; Biotechnology; Cancer; Immunology; Microbiology; Nanocomplex.