Effects of nitrogen fertilizers on the bacterial community diversity and the weathering of purple mudstone in Southwest China

Chunpei Li; Maopan Fan; Xuan Wang; Xue Li; Guang Zhao; Gangcai Liu; Jixia Zhao

doi:10.3389/fmicb.2023.1164826

Effects of nitrogen fertilizers on the bacterial community diversity and the weathering of purple mudstone in Southwest China

Front Microbiol. 2023 Jun 29:14:1164826. doi: 10.3389/fmicb.2023.1164826. eCollection 2023.

Authors

Chunpei Li¹, Maopan Fan¹, Xuan Wang^{2

3}, Xue Li¹, Guang Zhao⁴, Gangcai Liu², Jixia Zhao¹

Affiliations

¹ College of Resources and Environment, Yunnan Agricultural University, Kunming, China.
² Key Laboratory of Mountain Surface Processes and Ecological Regulation, Chinese Academy of Sciences, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, and Ministry of Water Conservancy, Chengdu, China.
³ University of the Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing, China.
⁴ Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, China.

Abstract

Introduction: Rock weathering is crucial in the development of soil. Yet the role of bacteria in the fine particle-forming process of purple mudstone is not fully understood, especially under nitrogen fertilization.

Methods: In this study, the particles (0.25 mm to 1 mm) of purple mudstone from Penglai Group (J₃p) were selected as the test material. Two nitrogen fertilizers, i.e., urea (U) and ammonium bicarbonate (AB), and four application levels (0, 280, 560, and 840 N kg∙ha^-1) with 18 replications were designed in an incubation experiment. The weathering indices and bacterial community structure of the purple mudstone particles were investigated after 120 days of incubation.

Results: The results showed that the weathering indices of purple mudstone particles in the AB treatment were higher than that in the U treatment at the same fertilization levels and a reducing trend was observed with increasing nitrogen fertilizer levels under the same nitrogen fertilizer application types. The diversities of the bacterial community were extremely significantly altered by nitrogen fertilizer application (p < 0.01). The effect of the nitrogen fertilizer application level on the beta diversity of the bacterial community (R² = 0.34) was greater than that of the nitrogen fertilizer application type (R² = 0.20). Through stepwise regression analysis, the positive effects of nitrification of Nitrobacter (Nitrolancea) (R² = 0.36), the Phosphorous-dissolving bacteria (Massilia) (R² = 0.12), and N-NO₃^- (R² = 0.35) on the weathering indices of J₃p purple mudstone particles could be observed. Structural equation modelling indicated that nitrogen fertilizer application level affects the abundance of the dominant species at the genus level (Nitrolancea and Massilia), and key environmental factor (N-NO₃^-), which in turn accelerated the weathering indices (59%).

Discussion and conclusion: Our findings imply that the enhancements of nitrification of Nitrobacter (Nitrolancea) and of phosphorus solubilization of Phosphorous-dissolving bacteria (Massilia) by nitrogen fertilization are the key factors affecting the weathering indices of J₃p purple mudstone particles.

Keywords: ammonium bicarbonate; bacterial community structure; purple mudstone weathering; urea; weathering indices.