Bovine muscle satellite cells in calves and cattle: A comparative study of cellular and genetic characteristics for cultivated meat production

Bosung Kim; Deunsol Ko; Seong Ho Choi; Sungkwon Park

doi:10.1016/j.crfs.2023.100545

Bovine muscle satellite cells in calves and cattle: A comparative study of cellular and genetic characteristics for cultivated meat production

Curr Res Food Sci. 2023 Jul 5:7:100545. doi: 10.1016/j.crfs.2023.100545. eCollection 2023.

Authors

Bosung Kim¹, Deunsol Ko¹, Seong Ho Choi², Sungkwon Park¹

Affiliations

¹ Sejong University, Department of Food Science and Biotechnology, Seoul, 05006, South Korea.
² Chungbuk National University, Department of Animal Science, Cheongju, 28644, South Korea.

Abstract

This study compared the cellular and genetic characteristics of bovine skeletal muscle satellite cells (SMSCs) from Hanwoo (a Korean native cattle breed), including calves and mature cattle. SMSCs were isolated using magnetic-activated cell sorting (MACS) from tissue samples of six Hanwoo (three calves and three mature cattle) using the CD29 antibody. Calves' SMSCs exhibited significantly faster growth rates than did those from cattle (P < 0.01), with a doubling time of 2.43 days. Genetic analysis revealed higher MyoD and Pax7 expression in SMSCs from calves during proliferation than in those from mature cattle (P < 0.001). However, FASN and PLAG1 expression levels were higher in mature cattle than in calves during both proliferation and differentiation (P < 0.001). These findings highlight the need for strategies to improve bovine muscle cell growth to produce competitive cultivated meat at a competitive price.

Keywords: Bovine muscle satellite cell; Cell growth; Genetic analysis; Primary culture.