Blueberries are rich in phenolic compounds including anthocyanins which are closely related to biological health functions. The purpose of this study was to investigate the antioxidant activity of blueberry anthocyanins extracted from 'Brightwell' rabbiteye blueberries in mice. After one week of adaptation, C57BL/6J healthy male mice were divided into different groups that were administered with 100, 400, or 800 mg/kg blueberry anthocyanin extract (BAE), and sacrificed at different time points (0.1, 0.5, 1, 2, 4, 8, or 12 h). The plasma, eyeball, intestine, liver, and adipose tissues were collected to compare their antioxidant activity, including total antioxidant capacity (T-AOC), superoxide dismutase (SOD) activity and glutathione-peroxidase (GSH-PX/GPX) content, and the oxidative stress marker malondialdehyde (MDA) level. The results showed that blueberry anthocyanins had positive concentration-dependent antioxidant activity in vivo. The greater the concentration of BAE, the higher the T-AOC value, but the lower the MDA level. The enzyme activity of SOD, the content of GSH-PX, and messenger RNA (mRNA) levels of Cu,Zn-SOD, Mn-SOD, and GPX all confirmed that BAE played an antioxidant role after digestion in mice by improving their antioxidant defense. The in vivo antioxidant activity of BAE indicated that blueberry anthocyanins could be developed into functional foods or nutraceuticals with the aim of preventing or treating oxidative stress-related diseases.
蓝莓富含花青素等酚类化合物，具有良好的保健功能。本研究旨在研究从‘灿烂’品种兔眼蓝莓中提取的蓝莓花青素在小鼠体内的抗氧化活性。C57BL/6J健康雄性小鼠被分成不同剂量组，各组蓝莓花青素提取物（BAE）浓度分别为100、400和800 mg/kg。在不同时间点（0.1、0.5、1、2、4、8和12 h）将小鼠处死，收集其血浆、眼球、肠、肝和脂肪组织，比较其抗氧化活性，测定指标包括总抗氧化能力（T-AOC），抗氧化酶如超氧化物歧化酶（SOD）活性和谷胱甘肽过氧化物酶（GSH-PX/GPX）含量，以及氧化应激标志物丙二醛（MDA）水平。结果表明，蓝莓花青素在小鼠体内具有良好的抗氧化活性，并呈浓度依赖性：BAE浓度越高，T-AOC值越高，MDA水平越低。抗氧化酶SOD活性、GSH-PX含量以及Cu,Zn-SOD、Mn-SOD和GPX的mRNA水平均证实，BAE在小鼠消化后通过改善其抗氧化防御系统发挥抗氧化作用。蓝莓花青素提取物的体内抗氧化活性表明，蓝莓花青素可以被开发成功能性食品或营养配料，用于预防或治疗氧化应激相关的疾病。.
蓝莓富含花青素等酚类化合物，具有良好的保健功能。本研究旨在研究从‘灿烂’品种兔眼蓝莓中提取的蓝莓花青素在小鼠体内的抗氧化活性。C57BL/6J健康雄性小鼠被分成不同剂量组，各组蓝莓花青素提取物（BAE）浓度分别为100、400和800 mg/kg。在不同时间点（0.1、0.5、1、2、4、8和12 h）将小鼠处死，收集其血浆、眼球、肠、肝和脂肪组织，比较其抗氧化活性，测定指标包括总抗氧化能力（T-AOC），抗氧化酶如超氧化物歧化酶（SOD）活性和谷胱甘肽过氧化物酶（GSH-PX/GPX）含量，以及氧化应激标志物丙二醛（MDA）水平。结果表明，蓝莓花青素在小鼠体内具有良好的抗氧化活性，并呈浓度依赖性：BAE浓度越高，T-AOC值越高，MDA水平越低。抗氧化酶SOD活性、GSH-PX含量以及Cu,Zn-SOD、Mn-SOD和GPX的mRNA水平均证实，BAE在小鼠消化后通过改善其抗氧化防御系统发挥抗氧化作用。蓝莓花青素提取物的体内抗氧化活性表明，蓝莓花青素可以被开发成功能性食品或营养配料，用于预防或治疗氧化应激相关的疾病。
Keywords: Blueberry anthocyanin; Glutathione-peroxidase (GSH-PX/GPX); In vivo antioxidant activity; Malondialdehyde (MDA); Superoxide dismutase (SOD).