Comprehensive understanding of enzymatic saccharification of Betaine:Lactic acid-pretreated sugarcane bagasse

Ying Chen; Cuiluan Ma; Wei Tang; Yu-Cai He

doi:10.1016/j.biortech.2023.129485

Comprehensive understanding of enzymatic saccharification of Betaine:Lactic acid-pretreated sugarcane bagasse

Bioresour Technol. 2023 Oct:386:129485. doi: 10.1016/j.biortech.2023.129485. Epub 2023 Jul 16.

Authors

Ying Chen¹, Cuiluan Ma², Wei Tang¹, Yu-Cai He³

Affiliations

¹ School of Pharmacy & School of Biological and Food Engineering, National-Local Joint Engineering Research Center of Biomass Refining and High-Quality Utilization, Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, Changzhou University, Changzhou 213164, PR China.
² State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Lifes, Hubei University, Wuhan 430062, PR China.
³ School of Pharmacy & School of Biological and Food Engineering, National-Local Joint Engineering Research Center of Biomass Refining and High-Quality Utilization, Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, Changzhou University, Changzhou 213164, PR China; State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Lifes, Hubei University, Wuhan 430062, PR China. Electronic address: heyucai2001@163.com.

PMID: 37454960
DOI: 10.1016/j.biortech.2023.129485

Abstract

Green solvents, especially deep eutectic solvents (DESs), are widely applied to pretreat biomass for enhancing its enzymatic hydrolysis. In this work, lactic acid was selected as the hydrogen-bond-donor to prepare Betaine-base DES (Betaine:LA), The DES was utilized to pretreat sugarcane bagasse (SCB) at 160 ℃ for 80 min (severity factor LogR_{0 =} 3.67). The influences of Betaine:LA treatment on the chemical composition, crystal and microstructure structure of cellulose, and cellulase digestion were investigated. The results showed that the lignin (47.1%) and xylan (44.6%) were removed, the cellulase digestibility of Betaine:LA-treated SCB was 4.2 times that of the raw material. This improved efficiency was attributed to the enhanced accessibility of cellulose, the weakened surface area of lignin, the declined hydrophobicity, and the decreased crystallinity of cellulose. Several compelling linear correlations were fitted between enzymatic hydrolysis and these alterations of physicochemical features, comprehensively understanding enzymatic saccharification of Betaine:LA-pretreated SCB.

Keywords: Accessibility; Enzymatic saccharification; Severity factor; Sugarcane bagasse pretreatment.

MeSH terms

Betaine
Cellulase* / chemistry
Cellulose / chemistry
Hydrolysis
Lactic Acid
Lignin / chemistry
Saccharum* / chemistry

Substances

bagasse
Cellulose
Lignin
Betaine
Lactic Acid
Cellulase