Understanding acid hydrolysis of corn stover during densification pretreatment for quantitative predictions of enzymatic hydrolysis efficiency using modified pretreatment severity factor

Abstract

DLCA(sa) pretreatment (densifying lignocellulosic biomass with sulfuric acid followed by autoclave treatment), featured with low treatment temperature and densification, demonstrate high efficiency in biomass pretreatment. In this study, the effects of temperature, acid loading, time on the hydrolysis of xylan, cellulose and lignin during DLCA(sa) pretreatment were systematically investigated. It was shown that DLCA(sa) pretreatment can effectively solubilize xylan, achieving an 84% xylose recovery under mild conditions (130 °C, 30 min, and 0.125 g/g acid loading). The conventional pretreatment severity factor correlated and further modified to improve the accuracy in evaluating the xylan hydrolysis. Additionally, a mathematical model based on the xylan hydrolytic kinetics was proposed to predict the enzymatic hydrolysis. Kinetic model suggested that mechanical densification facilitates the penetration of acid into the biomass matrix, leading to increased accessibility of xylan to acid catalysis.

Keywords: DLCA pretreatment; Enzymatic hydrolysis; Hemicellulose depolymerization; Lignocellulose.