Comparative analysis of toxicity and metabolomic profiling of rac-glufosinate and L-glufosinate in zebrafish

Abstract

Glufosinate is a chiral pesticide, with commercial formulations such as racemic glufosinate (rac-glufosinate) and pure L-glufosinate enantiomer (L-glufosinate) on the market. There has been little research on the difference in toxicity to non-target organisms between these two main ingredients. The effects of rac-glufosinate and L-glufosinate on glutathione reductase (GR), catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA) levels in zebrafish were investigated in this study. The effect of two glufosinate agents at low concentrations (0.01 and 0.1 mg/L) on these four oxidative indicators was found to be significantly lower than that of high concentrations (1 and 10 mg/L). L-glufosinate had a stronger enhancing effect on CAT, GR, and MDA content than rac-glufosinate and a stronger inhibitory effect on SOD activity than rac-glufosinate. The researchers used ultra-high-performance liquid chromatography coupled with high-resolution mass spectroscopy metabolomics to compare rac-glufosinate and L-glufosinate for metabolic disorders in adult zebrafish. Stable and obvious metabolic maps of the two agents were obtained using multivariate statistical results, such as principal component analysis and orthogonal partial minimum discriminant analysis. Compared to the control group, the rac-glufosinate and L-glufosinate treatment groups shared 151 differential metabolites, which primarily affected zebrafish energy metabolism, amino acid metabolism, and other metabolic pathways. Caffeine metabolism and biotin metabolism were among the unique pathways disrupted in rac-glufosinate-exposed zebrafish. Contrarily, L-glufosinate treatment primarily affected eight metabolic pathways, including arginine biosynthesis, melanogenesis, and glutathione metabolism. These findings may provide more detailed information on the toxicity of rac-glufosinate and L-glufosinate in zebrafish, as well as some context for assessing the environmental risk of the two glufosinate agents to aquatic organisms.

Keywords: L-glufosinate; Metabolomic; Oxidative Damage; Rac-glufosinate; Zebrafish.