Cataloguing Protein Complexes In Planta Using TurboID-Catalyzed Proximity Labeling

Lore Gryffroy; Joren De Ryck; Veronique Jonckheere; Sofie Goormachtig; Alain Goossens; Petra Van Damme

doi:10.1007/978-1-0716-3327-4_26

Cataloguing Protein Complexes In Planta Using TurboID-Catalyzed Proximity Labeling

Methods Mol Biol. 2023:2690:311-334. doi: 10.1007/978-1-0716-3327-4_26.

Authors

Lore Gryffroy^{1

2

3}, Joren De Ryck^{1

2

3}, Veronique Jonckheere³, Sofie Goormachtig^{1

2}, Alain Goossens^{1

2}, Petra Van Damme⁴

Affiliations

¹ Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
² Center for Plant Systems Biology, Ghent, Belgium.
³ iRIP Unit, Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium.
⁴ iRIP Unit, Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium. petra.vandamme@ugent.be.

PMID: 37450157
DOI: 10.1007/978-1-0716-3327-4_26

Abstract

Mapping protein-protein interactions is crucial to understand protein function. Recent advances in proximity-dependent biotinylation (BioID) coupled to mass spectrometry (MS) allow the characterization of protein complexes in diverse plant models. Here, we describe the use of BioID in hairy root cultures of tomato and provide detailed information on how to analyze the data obtained by MS.

Keywords: Interactomics; Mass spectrometry; Protein; Protein interactions; Proximity-dependent biotinylation; Solanaceae hairy root cultures; TurboID.

MeSH terms

Biotinylation
Catalysis
Protein Interaction Mapping* / methods
Proteins*

Substances

Proteins