Liquid biopsy has obvious advantages over invasive sampling methods. Cell-free DNA is now widely used in prenatal and cancer diagnosis. Cell-free RNA is relatively unstable but could contain potential biomarkers, and some studies have demonstrated that cfRNA sequencing may be developed to be a promising diagnostic assay for disease detection. In this chapter, we introduce a modified protocol for the detection of cfRNA based on next-generation sequencing (NGS). This protocol has been specifically optimized for low-input, fragmented cfRNA samples.
Keywords: Liquid biopsy; Low-abundant RNA library construction; cfRNA biomarkers; cfRNA sequencing.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.