Responsive Janus droplets as modular sensory layers for the optical detection of bacteria

Abstract

The field of biosensor development is fueled by innovations in new functional transduction materials and technologies. Material innovations promise to extend current sensor hardware limitations, reduce analysis costs, and ensure broad application of sensor methods. Optical sensors are particularly attractive because they enable sensitive and noninvasive analyte detection in near real-time. Optical transducers convert physical, chemical, or biological events into detectable changes in fluorescence, refractive index, or spectroscopic shifts. Thus, in addition to sophisticated biochemical selector designs, smart transducers can improve signal transmission and amplification, thereby greatly facilitating the practical applicability of biosensors, which, to date, is often hampered by complications such as difficult replication of reproducible selector-analyte interactions within a uniform and consistent sensing area. In this context, stimuli-responsive and optically active Janus emulsions, which are dispersions of kinetically stabilized biphasic fluid droplets, have emerged as a novel triggerable material platform that provides as a versatile and cost-effective alternative for the generation of reproducible, highly sensitive, and modular optical sensing layers. The intrinsic and unprecedented chemical-morphological-optical coupling inside Janus droplets has facilitated optical signal transduction and amplification in various chemo- and biosensor paradigms, which include examples for the rapid and cost-effective detection of major foodborne pathogens. These initial demonstrations resulted in detection limits that rival the capabilities of current commercial platforms. This trend article aims to present a conceptual summary of these initial efforts and to provide a concise and comprehensive overview of the pivotal kinetic and thermodynamic principles that govern the ability of Janus droplets to sensitively and selectively respond to and interact with bacteria.

Keywords: Biosensors; Emulsions; Foodborne pathogens; Responsive materials; Signal transduction; Stimuli-responsive surfactants.