Enhanced effects of antagomiR-3074-3p-conjugated PEI-AuNPs on the odontogenic differentiation by targeting FKBP9

Tao Jiang; Shenghong Miao; Jingjie Shen; Wenjing Song; Shenglong Tan; Dandan Ma

doi:10.1177/20417314231184512

Enhanced effects of antagomiR-3074-3p-conjugated PEI-AuNPs on the odontogenic differentiation by targeting FKBP9

J Tissue Eng. 2023 Jun 29:14:20417314231184512. doi: 10.1177/20417314231184512. eCollection 2023 Jan-Dec.

Authors

Tao Jiang¹, Shenghong Miao^{2

3}, Jingjie Shen⁴, Wenjing Song⁴, Shenglong Tan¹, Dandan Ma¹

Affiliations

¹ Department of Endodontics, Stomatological Hospital, School of Stomatology, Southern Medical University, Guangzhou, China.
² College of Stomatology, Southern Medical University, Guangzhou, China.
³ Foshan Stomatological Hospital, Foshan University, Foshan, Guangdong, China.
⁴ School of Material Science and Engineering, South China University of Technology, Guangzhou, China.

Abstract

The odontogenic differentiation of dental pulp stem cells (DPSCs), which is vital for tooth regeneration, was regulated by various functional molecules. In recent years, a growing body of research has shown that miRNAs play a crucial role in the odontogenic differentiation of human dental pulp stem cells (hDPSCs). However, the mechanisms by which miRNAs regulated odontogenic differentiation of hDPSCs remained unclear, and the application of miRNAs in reparative dentin formation in vivo was also rare. In this study, we first discovered that miR-3074-3p had an inhibitory effect on odontogenic differentiation of hDPSCs and antagomiR-3074-3p-conjugated PEI-AuNPs effectively promoted odontogenic differentiation of hDPSCs in vitro. AntagomiR-3074-3p-conjugated PEI-AuNPs was further applied to the rat pulp-capping model and showed the increased formation of restorative dentin. In addition, the results of lentivirus transfection in vitro suggested that FKBP9 acted as the key target of miR-3074-3p in regulating the odontogenic differentiation of hDPSCs. These findings might provide a new strategy and candidate target for dentin restoration and tooth regeneration.

Keywords: Dental pulp stem cell; FKBP9; miR-3074-3p; odontogenic differentiation; tooth regeneration.