Protective effect of Salvia hispanica (chia seeds) against obesity induced ovarian disorders in rat model

Eman Mahmoud FaragAllah; Samah Attia Algharib; Nehal I A Goda; Nanees F El-Malkey; Heba H Mahboub; Shaimaa H El-Fayoumi; Nawal Al-Hoshani; Reham M Wahid; Mohamed Aref

doi:10.1016/j.tice.2023.102156

Protective effect of Salvia hispanica (chia seeds) against obesity induced ovarian disorders in rat model

Tissue Cell. 2023 Aug:83:102156. doi: 10.1016/j.tice.2023.102156. Epub 2023 Jul 5.

Authors

Eman Mahmoud FaragAllah¹, Samah Attia Algharib², Nehal I A Goda³, Nanees F El-Malkey¹, Heba H Mahboub⁴, Shaimaa H El-Fayoumi⁵, Nawal Al-Hoshani⁶, Reham M Wahid¹, Mohamed Aref⁷

Affiliations

¹ Physiology Department, Faculty of Medicine, Zagazig University, 44511 Zagazig, Sharkia, Egypt.
² Department of Clinical Pathology, Faculty of Veterinary Medicine, Benha University, Moshtohor, 13736 Toukh, QG, Egypt; National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues, China.
³ Department of Histology and Cytology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia, Egypt.
⁴ Department of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia, Egypt. Electronic address: hhhmb@yahoo.com.
⁵ Department of Pharmacology & Toxicology, Faculty of Pharmacy, Heliopolis University, Egypt.
⁶ Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
⁷ Anatomy Department, Faculty of Veterinary Medicine, Zagazig University, 44511 Zagazig, Sharkia, Egypt.

PMID: 37437332
DOI: 10.1016/j.tice.2023.102156

Abstract

The main goal of the current report is to assess the protective impacts of chia seeds against obesity-induced ovarian dysfunctions with a trial to elucidate the mechanism of action. Forty rats were divided into 4 groups including lean untreated, lean consuming chia seeds, obese untreated, and rats consumed high-fat diet (HFD) mixed with ground chia seeds for 10 weeks. Anthropometric measures including visceral fat, peri-ovarian fat, ovarian weights, and duration of the estrous cycle were computed. Serum luteinizing (LH), follicular stimulating (FSH), progesterone, estradiol hormones, and tumor necrosis-α (TNF-α) were estimated. Ovarian histopathology and immunohistochemistry (CD31) were performed. Results showed that chia seeds clearly reduced obesity and induced alteration in anthropometric measures with a clear increase in LH and progesterone. Such seeds notably reversed histopathological alteration and reduced TNF-α, and CD31 induced by HFD. Conclusively, chia seeds have a potential protective role against obesity-induced ovarian dysfunction owing to their anti-inflammatory properties.

Keywords: Biochemical analysis; Chia seeds; Histopathology; Immunohistochemistry; Obesity; Ovarian function.

MeSH terms

Animals
Obesity / complications
Progesterone
Rats
Salvia hispanica*
Salvia* / chemistry
Seeds / chemistry
Tumor Necrosis Factor-alpha

Substances

Salvia hispanica seed extract
Tumor Necrosis Factor-alpha
Progesterone