The DEAD-box RNA helicase Dbp5 is a key protein that couples multiple steps in gene expression

Biol Chem. 2023 Jul 13;404(8-9):845-850. doi: 10.1515/hsz-2023-0130. Print 2023 Jul 26.

Abstract

Cell viability largely depends on the surveillance of mRNA export and translation. Upon pre-mRNA processing and nuclear quality control, mature mRNAs are exported into the cytoplasm via Mex67-Mtr2 attachment. At the cytoplasmic site of the nuclear pore complex, the export receptor is displaced by the action of the DEAD-box RNA helicase Dbp5. Subsequent quality control of the open reading frame requires translation. Our studies suggest an involvement of Dbp5 in cytoplasmic no-go-and non-stop decay. Most importantly, we have also identified a key function for Dbp5 in translation termination, which identifies this helicase as a master regulator of mRNA expression.

Keywords: Dbp5/DDX19; NGD; NSD; mRNA export; mRNA quality control; translation termination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus
  • DEAD-box RNA Helicases / genetics
  • DEAD-box RNA Helicases / metabolism
  • Gene Expression
  • Nucleocytoplasmic Transport Proteins* / genetics
  • Nucleocytoplasmic Transport Proteins* / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Saccharomyces cerevisiae Proteins* / metabolism

Substances

  • Nucleocytoplasmic Transport Proteins
  • DEAD-box RNA Helicases
  • Saccharomyces cerevisiae Proteins
  • RNA, Messenger