Exposure to silver and titanium dioxide nanoparticles at supra-environmental concentrations decreased sperm motility and affected spermatozoa subpopulations in gilthead seabream, Sparus aurata

Catarina C V Oliveira; Leonor Ferrão; Victor Gallego; Cláudia Mieiro; Isabel B Oliveira; Ana Carvalhais; Mário Pachedo; Elsa Cabrita

doi:10.1007/s10695-023-01218-0

Exposure to silver and titanium dioxide nanoparticles at supra-environmental concentrations decreased sperm motility and affected spermatozoa subpopulations in gilthead seabream, Sparus aurata

Fish Physiol Biochem. 2023 Jul 12. doi: 10.1007/s10695-023-01218-0. Online ahead of print.

Authors

Catarina C V Oliveira¹, Leonor Ferrão^{2

3}, Victor Gallego^{2

3}, Cláudia Mieiro⁴, Isabel B Oliveira⁴, Ana Carvalhais⁴, Mário Pachedo⁴, Elsa Cabrita²

Affiliations

¹ Centre of Marine Sciences (CCMAR), University of Algarve, 8005-139, Faro, Portugal. ccoliveira@ualg.pt.
² Centre of Marine Sciences (CCMAR), University of Algarve, 8005-139, Faro, Portugal.
³ Aquaculture and Biodiversity Group, Universitat Politècnica de València, 46022, València, Spain.
⁴ Centre for Environmental and Marine Studies (CESAM) and Department of Biology, University of Aveiro, 3810-193, Aveiro, Portugal.

PMID: 37436567
DOI: 10.1007/s10695-023-01218-0

Abstract

Marine pollution by nanoparticles (NPs) can be reprotoxic for fish and disturb successful reproduction of wild populations. In gilthead seabream (Sparus aurata), a mild effect on sperm motility was observed after exposure to high concentrations of silver NPs. Considering the great heterogeneity traits within a sperm sample, it is possible that NPs affect spermatozoa accordingly, modulating subpopulation profile. Thus, this work aimed to analyse NP effects in sperm motility in general and considering spermatozoa population structure, using a subpopulation approach. Seabream sperm samples from mature males were exposed for 1 h to increasing concentrations of titanium dioxide (1, 10, 100, 1000 and 10,000 μg L^-1) and silver (0.25, 25 and 250 μg L^-1) NPs, including Ag NP and Ag⁺, dissolved in a non-activating medium (0.9 % NaCl). Concentrations chosen include realistic (10-100 and 0.25 μg L^-1, respectively, for TiO₂ and Ag) and supra-environmental values. The mean particle diameter was determined as 19.34 ± 6.72 and 21.50 ± 8.27 nm in the stock suspension, respectively, for titanium dioxide and silver. After the ex vivo exposure, sperm motility parameters were determined using computer-assisted sperm analysis, and sperm subpopulations were later identified using a two-step cluster analysis. Results revealed a significant reduction in total motility after exposure to the 2 highest concentrations of titanium dioxide NPs, while curvilinear and straight-line velocities were not altered. Exposure to silver NPs (Ag NP and Ag⁺) lowered significantly total and progressive motilities at all concentrations, while curvilinear and straight-line velocities were significantly lower only at the highest concentration. Sperm subpopulations were also affected by the exposure to both titanium dioxide and silver NPs. In both cases, the highest levels of NPs triggered a decrease in the percentage of fast sperm subpopulations (38.2% in TiO₂ 1000 μg L^-1, 34.8.% in Ag NP 250 μg L^-1, and 45.0% in Ag⁺ 250 μg L^-1 vs 53.4% in the control), while an increase on slow sperm subpopulations. A reprotoxic effect was proven for both NPs, but only at supra-environmental concentrations.

Keywords: Ex vivo exposure; Nanoparticles; Reprotoxicity; Sperm subpopulations.